Lombardo Marco, Serrao Sebastiano, Carbone Giovanni, Lombardo Giuseppe
From the G.B. Bietti Foundation-IRCCS (M. Lombardo, Serrao) and Vision Engineering Italy srl (G. Lombardo), Rome, and the CNR-IPCF Unit of Support of Cosenza (Carbone, G. Lombardo), Rende, Italy.
From the G.B. Bietti Foundation-IRCCS (M. Lombardo, Serrao) and Vision Engineering Italy srl (G. Lombardo), Rome, and the CNR-IPCF Unit of Support of Cosenza (Carbone, G. Lombardo), Rende, Italy.
J Cataract Refract Surg. 2015 Mar;41(3):635-43. doi: 10.1016/j.jcrs.2014.07.031.
To analyze the spatial distribution and time course of corneal light backscattering before and after transepithelial corneal crosslinking using iontophoresis.
Fondazione G.B. Bietti-IRCCS, Rome, Italy.
Experimental study.
Three donor human eyes with an intact corneal epithelium had transepithelial iontophoresis corneal crosslinking (using rapid ultraviolet-A [UVA] irradiation), and 3 donor eyes without corneal epithelium had standard corneal crosslinking (using standard UVA irradiation). In addition, 3 donor eyes had iontophoresis and rapid corneal crosslinking after corneal deepithelialization (epi-off iontophoresis corneal crosslinking). Scheimpflug images (Pentacam HR) of each eye globe were acquired before and immediately after administration of riboflavin 0.1% solutions and 5, 10, 30, and 120 minutes after the corneal crosslinking procedures. Corneal light backscattering was quantified across the anterior 280 μm thickness at several points from the optical center to 3.0 mm from the center.
Light backscattering significantly increased after iontophoresis (P < .001) in specimens with and without intact epithelium. It decreased significantly after transepithelial iontophoresis corneal crosslinking and epi-off iontophoresis corneal crosslinking (P < .001), approaching the baseline values. After standard stromal soaking with riboflavin, a large increase in corneal light backscattering was found compared with baseline measurements (P < .001) that remained unchanged up to 30 minutes after standard corneal crosslinking (P = .92). The light backscattering increase after iontophoresis in corneas with epithelium was lower than after standard soaking (P = .01). No differences were found between specimens without epithelium after iontophoresis and standard stromal soaking (P = .06).
Scheimpflug photography provided an indirect biomarker of stromal permeation of riboflavin. Iontophoresis efficiently delivered riboflavin through the epithelium.
No author has a financial or proprietary interest in any material or method mentioned.
分析采用离子电渗法的经上皮角膜交联术前、后角膜光背散射的空间分布及时间进程。
意大利罗马的G.B. 比耶蒂基金会 - 意大利国家研究委员会眼科研究所。
实验研究。
3只角膜上皮完整的供体人眼接受经上皮离子电渗法角膜交联术（采用快速紫外线A [UVA]照射），3只无角膜上皮的供体眼接受标准角膜交联术（采用标准UVA照射）。此外，3只供体眼在角膜上皮去除后接受离子电渗法和快速角膜交联术（上皮去除后离子电渗法角膜交联术）。在每只眼球给予0.1%核黄素溶液前、给药后即刻以及角膜交联术后5、10、30和120分钟采集Scheimpflug图像（Pentacam HR）。在从光学中心到中心3.0 mm的几个点处，对前280μm厚度范围内的角膜光背散射进行定量分析。
在有和无完整上皮的标本中，离子电渗法后光背散射显著增加（P <.001）。经上皮离子电渗法角膜交联术和上皮去除后离子电渗法角膜交联术后光背散射显著降低（P <.001），接近基线值。在用核黄素进行标准基质浸润后，与基线测量值相比，角膜光背散射大幅增加（P <.001），在标准角膜交联术后30分钟内保持不变（P =.92）。有上皮的角膜在离子电渗法后光背散射的增加低于标准浸润后（P =.01）。离子电渗法后无上皮的标本与标准基质浸润后无差异（P =.06）。
Scheimpflug摄影提供了核黄素基质渗透的间接生物标志物。离子电渗法有效地将核黄素透过上皮输送。
没有作者对文中提及的任何材料或方法拥有财务或专利权益。
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