Department of Plant Molecular Physiology, Institute of Experimental Biology, University of Wrocław, Kanonia Street 6/8, 50-328 Wrocław, Poland.
Plant Sci. 2015 May;234:50-9. doi: 10.1016/j.plantsci.2015.02.005. Epub 2015 Feb 19.
The aim of this study was to investigate the effect of cadmium on plasma membrane (PM) NADPH oxidase activity in cucumber roots. Plants were treated with cadmium for 1, 3 or 6 days. Some of the plants after 3-day exposure to cadmium were transferred to a medium without the heavy metal for the next 3 days. Treatment of plants with cadmium for 6 days stimulated the activity of NADPH oxidase. The highest stimulation of O2(•-) production by NADPH oxidase was observed in post-stressed plants, which was correlated with the stimulation of activity of PM H(+)-ATPase in the same conditions. In order to examine the effects of cadmium stresses on the expression level of genes encoding NADPH oxidase, putative cucumber homologs encoding RBOH proteins were selected and a real-time PCR assay was performed. NADPH is a substrate for oxidase; thus alterations in the activity of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, NADP-isocitrate dehydrogenase and NADP-malic enzyme under cadmium stress were studied. The activity of NADPH dehydrogenases was increased under cadmium stress. The results indicate that PM NADPH oxidase could be involved in plants' response to cadmium stress by affecting the activity of PM H(+)-ATPase, and NADPH-generating enzymes could play important roles in this process.
本研究旨在探讨镉对黄瓜根系质膜 NADPH 氧化酶活性的影响。将植物用镉处理 1、3 或 6 天。一些植物在 3 天暴露于镉后被转移到不含重金属的培养基中，再过 3 天。6 天的镉处理刺激 NADPH 氧化酶的活性。在应激后的植物中观察到 NADPH 氧化酶产生 O2(•-)的最高刺激，这与在相同条件下 PM H(+)-ATPase 活性的刺激相关。为了研究镉胁迫对编码 NADPH 氧化酶的基因表达水平的影响，选择了编码 RBOH 蛋白的假定黄瓜同源物，并进行了实时 PCR 分析。NADPH 是氧化酶的底物；因此，研究了葡萄糖-6-磷酸脱氢酶、6-磷酸葡萄糖酸脱氢酶、NADP-异柠檬酸脱氢酶和 NADP-苹果酸酶在镉胁迫下的活性变化。在镉胁迫下，NADPH 脱氢酶的活性增加。结果表明，PM NADPH 氧化酶可能通过影响 PM H(+)-ATPase 的活性参与植物对镉胁迫的反应，而 NADPH 生成酶在这个过程中可能发挥重要作用。
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