对胰岛抗原有特异性的自身免疫性T淋巴细胞。

Autoimmune T-lymphocytes with specificity for pancreatic islet antigens.

作者信息

Kolb H, Ben-Nun A, Cohen I R, Barberena I, Kiesel U

出版信息

Immunol Lett. 1985;9(1):29-32. doi: 10.1016/0165-2478(85)90090-2.

Abstract

The question is still unresolved whether in insulin-dependent (type I) diabetes T-lymphocytes mediate an autoimmune response towards pancreatic islets. We now present direct evidence in an animal model for autoimmune T-lymphocytes with specificity for islet antigens. Mice of strain C57BL/6 were immunized repeatedly with islet homogenates. Lymphocytes isolated from draining lymph nodes and the spleen show a specific proliferative response when challenged with mouse rat or islet antigens but do not respond to liver or spleen antigens. Islet antigen specific lymphoblasts have been maintained by in vitro culture for several weeks in the presence of T-cell growth factor containing medium.

摘要

在胰岛素依赖型（I型）糖尿病中，T淋巴细胞是否介导针对胰岛的自身免疫反应这一问题仍未得到解决。我们现在在动物模型中提供了针对胰岛抗原的自身免疫性T淋巴细胞的直接证据。用胰岛匀浆反复免疫C57BL/6品系的小鼠。从引流淋巴结和脾脏分离出的淋巴细胞在受到小鼠、大鼠或胰岛抗原刺激时表现出特异性增殖反应，但对肝脏或脾脏抗原无反应。在含有T细胞生长因子的培养基存在下，胰岛抗原特异性淋巴母细胞已通过体外培养维持了数周。