Fu Yu, Maye Peter
Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, Connecticut.
Genesis. 2015 Mar-Apr;53(3-4):294-8. doi: 10.1002/dvg.22849. Epub 2015 Apr 11.
With the establishment of methods that provide evidence for the generation of chondrocyte and osteoblast cell types from ESCs, there is a need for reagents that will enable their further characterization. Here we report on the derivation of chondrocyte and osteoblast reporter ESCs from previously generated and characterized transgenic mouse lines, Collagen type 2 alpha 1(Col2a1)-ECFP, Bone Sialoprotein (BSP)-Topaz, and BSP-Topaz/Dentin Matrix Protein 1 (DMP1)-Cherry dual reporter mice. Col2a1-ECFP is highly expressed in chondrocytes, while BSP-Topaz and DMP1-Cherry are highly expressed in osteoblasts and osteocytes, respectively. These new skeletal reporter mouse ESC lines will serve as valuable reagents to investigate the functionality of ESC derived chondrocyte and osteoblast cell types.
随着能够为从胚胎干细胞生成软骨细胞和成骨细胞类型提供证据的方法的建立,需要能够对它们进行进一步表征的试剂。在此,我们报道了从先前生成并表征的转基因小鼠品系——Ⅱ型胶原α1(Col2a1)-增强型蓝色荧光蛋白(ECFP)、骨唾液酸蛋白(BSP)-黄玉、以及BSP-黄玉/牙本质基质蛋白1(DMP1)-樱桃双荧光报告基因小鼠——中获得软骨细胞和成骨细胞报告基因胚胎干细胞。Col2a1-ECFP在软骨细胞中高表达,而BSP-黄玉和DMP1-樱桃分别在成骨细胞和骨细胞中高表达。这些新的骨骼报告基因小鼠胚胎干细胞系将作为有价值的试剂,用于研究胚胎干细胞衍生的软骨细胞和成骨细胞类型的功能。