Shau H, Dawson J R
J Immunol. 1985 Jul;135(1):137-40.
The lysosome content of lymphocytes has been analyzed with lysosomotropic vital stains and the fluorescence-activated cell sorter (FACS). Large granular lymphocytes (LGL), which account for virtually all natural killing activity in peripheral blood, are quantitatively different from small lymphocytes (SL) in this respect. LGL obtained by Percoll gradient density centrifugation accumulate more of the lysosomotropic vital dyes than SL do, staining with either neutral red or mepacrine (quinacrine). Furthermore among the LGL-rich, low density lymphocyte population highly, granulated cells can be separated from less granulated ones by mepacrine staining and FACS. Thus, separated highly granulated LGL express very high natural killing, whereas the less granulated low density large lymphocytes do not kill.
已使用溶酶体亲和性活体染料和荧光激活细胞分选仪（FACS）分析了淋巴细胞的溶酶体含量。在这方面，几乎占外周血所有自然杀伤活性的大颗粒淋巴细胞（LGL）在数量上与小淋巴细胞（SL）不同。通过Percoll梯度密度离心获得的LGL比SL积累更多的溶酶体亲和性活体染料，用中性红或米帕林（奎纳克林）染色时均如此。此外，在富含LGL的低密度淋巴细胞群体中，高度颗粒化的细胞可以通过米帕林染色和FACS与颗粒较少的细胞分离。因此，分离出的高度颗粒化的LGL表现出非常高的自然杀伤活性，而颗粒较少的低密度大淋巴细胞则没有杀伤作用。
