Sakiyama S, Nakamura Y, Tokunaga K, Takazawa H, Ohwaki Y, Nagano T
Division of Biochemistry, Chiba Cancer Center Research Institute, Japan.
Cell Tissue Res. 1989 Nov;258(2):225-31. doi: 10.1007/BF00239442.
In-situ hybridization experiments have been performed using isoactin (beta and gamma)-specific riboprobes in various tissues of the rat and mouse. Distribution of the grains of actin mRNAs for both beta and gamma types was similar throughout sections of the rat testis. Although both mRNAs were evenly distributed in the seminiferous tubule, extremely heavy labeling was observed in about 10% of the seminiferous tubules that could be identified as stage XII of spermatogenesis. At high magnification, grains of the mRNA were found in the cytoplasm of elongating spermatids and in the Sertoli cell cytoplasm at the adluminal side. Much higher density of the grains of mRNA was observed in the neck region of the spermatids at stage XII. Thus, the dense distribution of cytoskeletal actin mRNAs is stage-specific in the tubule during spermatogenesis in the rat. The high expression of both beta and gamma actin mRNAs was also observed in the epithelial cells of the intestinal crypts.
已使用异肌动蛋白（β和γ）特异性核糖探针在大鼠和小鼠的各种组织中进行了原位杂交实验。在大鼠睾丸的各个切片中，β型和γ型肌动蛋白mRNA颗粒的分布相似。虽然两种mRNA在生精小管中分布均匀，但在约10%可鉴定为精子发生 XII 期的生精小管中观察到极强的标记。在高倍镜下，mRNA颗粒存在于伸长的精子细胞的细胞质以及近腔侧的支持细胞细胞质中。在 XII 期精子细胞的颈部区域观察到mRNA颗粒的密度要高得多。因此，在大鼠精子发生过程中，细胞骨架肌动蛋白mRNA的密集分布在生精小管中具有阶段特异性。在肠隐窝的上皮细胞中也观察到β和γ肌动蛋白mRNA的高表达。
