Jiang Qiao-hong, Gong Xue, Wang Xiao-xiang, He Fu-ming
Int J Oral Maxillofac Implants. 2015 Mar-Apr;30(2):461-71. doi: 10.11607/jomi.3798.
The aim of this study was to compare the promotion of osteogenesis in vitro on three types of titanium surfaces: a strontium-hydroxyapatite (Sr-HA)-coated surface, a nano-HA-coated surface, and an uncoated roughened surface.
Sr-HA and HA were placed on disks with a roughened titanium surface by electrochemical deposition. MC3T3-E1 preosteoblast cells and rat bone mesenchymal stem cells were cultured on the Sr-HA, HA-coated, and uncoated roughened disks, and cell adhesion, proliferation, viability, osteogenic differentiation, and formation of mineralized nodules were measured at various time points.
The Sr-HA coating produced by a simple electrochemical deposition treatment evidently enhanced the attachment, spreading, alkaline phosphatase activity, and extracellular matrix calcium mineralization of mouse bone mesenchymal stem cells and MC3T3-E1 cells compared with an untreated roughened titanium surface and a nano-HA-coated surface.
This study suggests that a Sr-doped nano-HA coating produced through electrochemical deposition improves the osteoconductivity of a microrough titanium surface.
本研究旨在比较三种类型钛表面（锶羟基磷灰石（Sr-HA）涂层表面、纳米羟基磷灰石（nano-HA）涂层表面和未涂层的粗糙表面）在体外促进成骨的情况。
通过电化学沉积将Sr-HA和HA置于具有粗糙钛表面的圆盘上。将MC3T3-E1前成骨细胞和大鼠骨髓间充质干细胞培养在Sr-HA、HA涂层和未涂层的粗糙圆盘上，并在不同时间点测量细胞黏附、增殖、活力、成骨分化和矿化结节的形成。
与未处理的粗糙钛表面和纳米HA涂层表面相比，通过简单的电化学沉积处理产生的Sr-HA涂层明显增强了小鼠骨髓间充质干细胞和MC3T3-E1细胞的附着、铺展、碱性磷酸酶活性以及细胞外基质钙矿化。
本研究表明，通过电化学沉积制备的掺锶纳米HA涂层可提高微粗糙钛表面的骨传导性。
