Boittin François-Xavier, Denis Josiane, Mayol Jean-François, Martigne Patrick, Raffin Florent, Coulon David, Grenier Nancy, Drouet Michel, Hérodin Francis
Department of Radiobiology, IRBA (Institut de Recherche Biomédicale des Armées), Brétigny-sur-Orge, France.
PLoS One. 2015 Apr 2;10(4):e0122900. doi: 10.1371/journal.pone.0122900. eCollection 2015.
In case of high-dose radiation exposure, mechanisms controlling late visceral organ damage are still not completely understood and may involve the central nervous system. To investigate the influence of cranial/brain irradiation on late visceral organ damage in case of high-dose exposure, Wistar rats were irradiated at 12 Gy, with either the head and fore limbs or the two hind limbs protected behind a lead wall (head- and hind limbs-protected respectively), which allows long-term survival thanks to bone marrow protection. Although hind limbs- and head-protected irradiated rats exhibited similar hematopoietic and spleen reconstitution, a late body weight loss was observed in hind limbs-protected rats only. Histological analysis performed at this time revealed that late damages to liver, kidney and ileum were attenuated in rats with head exposed when compared to animals whose head was protected. Plasma measurements of inflammation biomarkers (haptoglobin and the chemokine CXCL1) suggest that the attenuated organ damage in hind limbs-protected rats may be in part related to reduced acute and chronic inflammation. Altogether our results demonstrate the influence of cranial/brain exposure in the onset of organ damage.
在高剂量辐射暴露的情况下，控制晚期内脏器官损伤的机制仍未完全明确，可能涉及中枢神经系统。为了研究在高剂量暴露时颅脑照射对晚期内脏器官损伤的影响，对Wistar大鼠进行12 Gy的照射，分别用铅墙保护头部和前肢或两条后肢（分别为头部保护组和后肢保护组），由于骨髓得到保护，大鼠得以长期存活。尽管后肢保护组和头部保护组的辐照大鼠表现出相似的造血和脾脏重建，但仅在后肢保护组的大鼠中观察到晚期体重减轻。此时进行的组织学分析显示，与头部得到保护的动物相比，头部暴露的大鼠肝脏、肾脏和回肠的晚期损伤有所减轻。炎症生物标志物（触珠蛋白和趋化因子CXCL1）的血浆检测表明，后肢保护组大鼠器官损伤减轻可能部分与急性和慢性炎症减轻有关。总之，我们的结果证明了颅脑暴露对器官损伤发生的影响。
