Rukavtsova Elena B, Rudenko Natalya V, Puchko Elena N, Zakharchenko Natalya S, Buryanov Yaroslav I
Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Science Avenue, 6, Pushchino, Moscow Region 142290, Russia.
Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Science Avenue, 6, Pushchino, Moscow Region 142290, Russia.
J Biotechnol. 2015 Jun 10;203:84-8. doi: 10.1016/j.jbiotec.2015.03.019. Epub 2015 Mar 31.
Oral immunogenicity of the hepatitis B surface antigen (HBsAg) synthesized in the tubers of marker-free potato plants has been demonstrated. Experiments were performed in the two groups of outbred NMRI mice. At the beginning of investigations, the mice of experimental group were fed the tubers of transgenic potato synthesizing the HBsAg three times. The mice of control group were fed nontransgenic potato. Intraperitoneal injection of the commercial vaccine against hepatitis B (0.5μg/mouse) was made on day 71 of the experiment. Enzyme-linked immunoassay (ELISA) of the serum of immunized animals showed an increase in the level of HBsAg antibodies significantly above the protective value, which was maintained for 1 year after the immunization. In 1 year, the experimental group of mice underwent additional oral immunization with HBsAg-containing potato tubers. As a result, the level of antibodies against the HBsAg increased and remained at a high protective level for several months. The findings show the possibility of using transgenic plants as a substance for obtaining a safe edible vaccine against hepatitis B.
已证明在无标记马铃薯植株块茎中合成的乙型肝炎表面抗原（HBsAg）具有口服免疫原性。在两组远交NMRI小鼠中进行了实验。在研究开始时，给实验组小鼠喂食三次合成HBsAg的转基因马铃薯块茎。给对照组小鼠喂食非转基因马铃薯。在实验的第71天，对小鼠腹腔注射商用乙型肝炎疫苗（0.5μg/只）。对免疫动物血清进行酶联免疫吸附测定（ELISA）显示，HBsAg抗体水平显著高于保护值，并在免疫后维持了1年。1年后，对实验组小鼠用含HBsAg的马铃薯块茎进行额外的口服免疫。结果，抗HBsAg抗体水平升高，并在几个月内保持在高保护水平。这些发现表明，利用转基因植物作为一种物质来获得安全的乙型肝炎口服疫苗是有可能的。
