使用新型超声/近红外双模态造影剂对VX2肿瘤模型前哨淋巴结的检测及病理评估

Detection and Pathologic Evaluation of Sentinel Lymph Nodes in the VX2 Tumor Model Using a Novel Ultrasound/Near-Infrared Dual-Modality Contrast Agent.

作者信息

Qu Enze, Dai Zhifei, Liang Xiaolong, Qian Yajun, Wang Shumin, Ke Hengte, Wang Jinrui

机构信息

Department of Ultrasound, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Department of Life Sciences, Peking University Institute of Technology, Beijing, China.

出版信息

Ultrasound Med Biol. 2015 Jul;41(7):1905-12. doi: 10.1016/j.ultrasmedbio.2015.03.005. Epub 2015 Apr 1.

DOI:10.1016/j.ultrasmedbio.2015.03.005

PMID:25842255

Abstract

This study was conducted with the aim of developing a microbubble agent for near-infrared (NIR) fluorescence and ultrasound dual-modality contrast microbubbles applicable to imaging of sentinel lymph nodes in the VX2 rabbit tumor model. Specific ligands of phosphatidylserine (PS) and Cy7 NIR fluorescent dyes with long emission wavelengths (750-900 nm) were conjugated to the surface of ultrasound contrast microbubbles (MBs), termed Cy7 PS MBs. Ultrasound lymphography and NIR fluorescence imaging were performed using subcutaneous injection of Cy7 PS MBs to visualize the sentinel lymph node. Sentinel lymph node detection rates using the patent blue method, ultrasound lymphography and NIR fluorescence imaging were 95%, 79% and 95%, respectively, and sensitivity was 87%, 74% and 92%, respectively. With 2-D ultrasound, the diagnostic sensitivity for detection of sentinel lymph node metastases was 60% and the specificity was 74%, whereas Cy7 PS MB-enhanced ultrasound had a sensitivity of 80% and a specificity of 87%. The results indicate that dual-modality Cy7 PS MBs combined with ultrasound lymphography and NIR fluorescence may be useful in the detection of normal and metastasized sentinel lymph nodes.

摘要

本研究旨在开发一种用于近红外（NIR）荧光和超声双模态对比微泡的微泡剂，该微泡剂适用于VX2兔肿瘤模型中前哨淋巴结的成像。将磷脂酰丝氨酸（PS）的特异性配体和发射波长较长（750 - 900 nm）的Cy7近红外荧光染料偶联到超声对比微泡（MBs）表面，称为Cy7 PS MBs。通过皮下注射Cy7 PS MBs进行超声淋巴造影和近红外荧光成像，以可视化前哨淋巴结。使用专利蓝法、超声淋巴造影和近红外荧光成像的前哨淋巴结检测率分别为95%、79%和95%，敏感性分别为87%、74%和92%。二维超声检测前哨淋巴结转移的诊断敏感性为60%，特异性为74%，而Cy7 PS MB增强超声的敏感性为80%，特异性为87%。结果表明，双模态Cy7 PS MBs联合超声淋巴造影和近红外荧光可能有助于检测正常和转移的前哨淋巴结。