空间飞行后大肠杆菌的基因组和蛋白质组分析揭示了涉及代谢途径的变化。
Genomic and Proteomic Analysis of Escherichia coli After Spaceflight Reveals Changes Involving Metabolic Pathways.
机构信息
Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, Beijing, China.
Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, Beijing, China.
出版信息
Arch Med Res. 2015 Apr;46(3):181-5. doi: 10.1016/j.arcmed.2015.03.007. Epub 2015 Apr 3.
BACKGROUND AND AIMS
The space environment could have impacts on a variety of characteristics of microorganism such as cell metabolism, drug resistance, and virulence. However, relevant mechanisms need to be clarified. In the present study, the effect of a space environment on Escherichia coli was investigated.
METHODS
E. coli strains were sent to space for 398 h on the Shenzhou VIII and ground simulation was conducted as control. After the flight, a mutant strain LCT-EC67 was selected for further analysis.
RESULTS
Although no changes in hemolysis, morphology or antibiotic sensitivity were observed, the mutant strain showed elevated carbon source utilization compared with the control group. Genomic and proteomic analyses showed that 801 genes were upregulated and 825 genes were downregulated. In addition, 167 proteins were overexpressed and 92 proteins were downregulated using a cut-off fold-change value of 1.4 and a p < 0.05. The changed proteins were associated with metabolic functions such as alanine and glutamate metabolism, arginine and proline metabolism, and fatty acid and propanoate metabolism.
CONCLUSIONS
E. coli showed alterations at gene and protein levels mainly regarding biochemical metabolism after spaceflight.
背景与目的
太空环境可能对微生物的多种特性产生影响，如细胞代谢、耐药性和毒力。然而，相关机制仍需阐明。本研究旨在探讨太空环境对大肠杆菌的影响。
方法
将大肠杆菌菌株送往神舟八号进行 398 小时的太空飞行实验，并进行地面模拟作为对照。飞行后，选择一个突变株 LCT-EC67 进行进一步分析。
结果
尽管溶血、形态或抗生素敏感性没有变化，但突变株的碳源利用率比对照组有所提高。基因组和蛋白质组分析表明，801 个基因上调，825 个基因下调。此外，使用 1.4 的截止倍数变化值和 p < 0.05，有 167 个蛋白过表达，92 个蛋白下调。这些变化的蛋白与代谢功能有关，如丙氨酸和谷氨酸代谢、精氨酸和脯氨酸代谢以及脂肪酸和丙酸代谢。
结论
大肠杆菌在太空飞行后，基因和蛋白质水平主要表现为生化代谢的改变。
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