Zhou Yan, Wu Zhencun, Tang Yuhong, Jia Tianjun
Department of Immunology, Hebei North University, Zhangjiakou 075000, China.
Department of Clinical Laboratory, Maternal and Child Health Hospital of Zhangjiakou City, Zhangjiakou 075000, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Apr;31(4):524-7.
To investigate the association of human leukocyte antigen-E (HLA-E) gene polymorphisms and plasma soluble HLA-E (sHLA-E) levels with genetic susceptibility to breast cancer in Zhangjiakou women of Han nationality.
HLA-E typing was performed in 200 patients with breast cancer and 228 healthy controls from Zhangjiakou area by means of polymerase chain reaction-sequence-specific priming (PCR-SSP). Simultaneously, sHLA-E was detected by ELISA.
We found two alleles of HLA-E: HLA-E0101 and HLA-E0103, and three genotypes, namely HLA-E0101/HLA-E0101, HLA-E0101/HLA-E0103 and HLA-E0103/HLA-E0103. The frequencies of HLA-E0103 allele and HLA-E0103/HLA-E0103 genotype showed a significant difference between healthy controls and patients with breast cancer. The HLA-E0103/HLA-E0103 genotype significantly increased the risk for breast cancer. Plasma levels of sHLA-E showed no significant difference in breast cancer patients and controls. While in breast cancer patients with HLA-E0103/HLA-E*0103 genotype, plasma levels of sHLA-E were significantly elevated compared with those in healthy controls. HLA-E genotypes and sHLA-E levels had no obvious relationship with tumor grading.
HLA-E gene polymorphisms are associated with the development of breast cancer in Zhangjiakou women of Han nationality. HLA-E0103/HLA-E0103 genotype is probably susceptible genotype for breast cancer, while plasma sHLA-E level probably has no association with breast cancer susceptibility.
探讨人类白细胞抗原-E（HLA-E）基因多态性及血浆可溶性HLA-E（sHLA-E）水平与张家口地区汉族女性乳腺癌遗传易感性的关系。
采用聚合酶链反应-序列特异性引物法（PCR-SSP）对张家口地区200例乳腺癌患者及228例健康对照者进行HLA-E分型。同时，采用酶联免疫吸附测定法（ELISA）检测sHLA-E。
发现HLA-E基因有两个等位基因：HLA-E0101和HLA-E0103，三种基因型，即HLA-E0101/HLA-E0101、HLA-E0101/HLA-E0103和HLA-E0103/HLA-E0103。HLA-E0103等位基因频率及HLA-E0103/HLA-E0103基因型频率在健康对照者与乳腺癌患者之间存在显著差异。HLA-E0103/HLA-E0103基因型显著增加乳腺癌发病风险。乳腺癌患者与对照者血浆sHLA-E水平无显著差异。而HLA-E0103/HLA-E*0103基因型的乳腺癌患者，其血浆sHLA-E水平较健康对照者显著升高。HLA-E基因型及sHLA-E水平与肿瘤分级无明显关系。
HLA-E基因多态性与张家口地区汉族女性乳腺癌的发生有关。HLA-E0103/HLA-E0103基因型可能是乳腺癌的易感基因型，而血浆sHLA-E水平可能与乳腺癌易感性无关。
