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峨眉角蟾(Megophrys omeimontis Liu,1950)(无尾目,角蟾科)近乎完整的线粒体基因组序列。

The near-complete mitogenome sequence of the Omei Horned Toad Megophrys omeimontis Liu, 1950 (Anura, Megophryidae).

作者信息

Liu Jiabin, Xue Rui, Wang Ye, Li Diyan, Yan Qigui, Yang Jiandong

机构信息

a College of Animal Science and Technology, Sichuan Agricultural University , Chengdu , China and.

b College of Veterinary Medicine, Sichuan Agricultural University , Chengdu , China.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2389-90. doi: 10.3109/19401736.2015.1028044. Epub 2015 Apr 9.

DOI:10.3109/19401736.2015.1028044
PMID:25856516
Abstract

The nearly complete mitogenome of Megophrys omeimontis is 17,013 bp in length, including 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes and a D-loop region, and it shares the identical gene arrangement with Atympanophrys shapingensis. The overall base composition of this mitogenome is biased toward AT content at 60.10%. Most PCGs begin with ATG, whereas ND1 starts with ATA, ND2 initiates from ATT, ND3 starts with ATC, and COX1 begins with GTG. Four PCGs (COX1, ATP8, ND4L and ND5) use complete stop codon TAR, and ND6 ends with AGG, but the remaining eight PCGs terminate in TA or T. The putative control region of M. omeimontis mitogenome holds two tandem repeat motifs at the 5' and 3' ends, which is different from that of A. shapingensis mtDNA. Megophrys omeimontis becomes the sister lineage to the two other species, B. carinense and A. shapingensis.

摘要

峨眉角蟾几乎完整的线粒体基因组长度为17,013 bp,包括13个蛋白质编码基因(PCGs)、2个rRNA基因、22个tRNA基因和一个D-loop区域,并且它与棘臂角蟾具有相同的基因排列。该线粒体基因组的总体碱基组成偏向于AT含量,为60.10%。大多数PCGs以ATG起始,而ND1以ATA起始,ND2从ATT起始,ND3以ATC起始,COX1以GTG起始。四个PCGs(COX1、ATP8、ND4L和ND5)使用完整的终止密码子TAR,ND6以AGG结尾,但其余八个PCGs以TA或T终止。峨眉角蟾线粒体基因组的假定控制区域在5'和3'端有两个串联重复基序,这与棘臂角蟾线粒体DNA不同。峨眉角蟾成为另外两个物种——隆肛蛙和棘臂角蟾的姐妹谱系。

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