Hong Shan-Chao, Lv Yuan, Fang Fu-Jin, Zhu Chang-Liang
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2014 Dec;26(6):672-4, 680.
To investigate the relationship between Wolbachia and deltamethrin resistance in Culex pipiens pallens.
PCR was used to detect Wolbachia in Culex pipiens pallens and qRT-PCR was performed to determine and compare the expression of Wolbachia between deltamethrin- resistant and - susceptible strains of Culex pipiens pallens.
Wolbachia was detected in Culex pipiens pallens in the laboratory. The expression of Wolbachia was 18.42, 3.69, 4.43, 3.96, 6.31, 1.55 and 3.76 folds higher in the deltamethrin-resistant strain than in susceptible strain in the egg, 1st, 2nd, 3rd, 4th stages, and male and female adults, but there was no statistical difference in the pupae stage. The expression of Wolbachia was 2.64 folds higher in deltamethrin-resistant females than in susceptible females which were caught in Jiangxinzhou of Nanjing.
Wolbachia is associated with deltamethrin resistance in Culex pipines pallens.
研究淡色库蚊中沃尔巴克氏体与溴氰菊酯抗性之间的关系。
采用聚合酶链反应（PCR）检测淡色库蚊中的沃尔巴克氏体，并进行实时定量聚合酶链反应（qRT-PCR）以测定和比较淡色库蚊溴氰菊酯抗性品系和敏感品系中沃尔巴克氏体的表达。
在实验室饲养的淡色库蚊中检测到了沃尔巴克氏体。在卵、1龄、2龄、3龄、4龄以及雌雄成虫阶段，溴氰菊酯抗性品系中沃尔巴克氏体的表达分别比敏感品系高18.42、3.69、4.43、3.96、6.31、1.55和3.76倍，但在蛹期无统计学差异。在南京江心洲捕获的溴氰菊酯抗性雌蚊中，沃尔巴克氏体的表达比敏感雌蚊高2.64倍。
沃尔巴克氏体与淡色库蚊的溴氰菊酯抗性有关。
