一种通过阻滞滚环扩增实现超灵敏检测凝血酶的等离子体适体传感器。
A plasmonic aptasensor for ultrasensitive detection of thrombin via arrested rolling circle amplification.
作者信息
Wang Sai, Bi Sai, Wang Zonghua, Xia Jianfei, Zhang Feifei, Yang Min, Gui Rijun, Li Yanhui, Xia Yanzhi
机构信息
College of Chemical Science and Engineering, Laboratory of Fiber Materials and Modern Textiles, The Growing Base for State Key Laboratory, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao 266071, P. R. China.
出版信息
Chem Commun (Camb). 2015 May 7;51(37):7927-30. doi: 10.1039/c5cc02069g.
A sensitive signal generation mechanism for gold nanoparticle growth by reducing gold ions with hydrogen peroxide is applied in a plasmonic aptasensor, achieving naked-eye detection of thrombin at the single-molecule level based on the specific interaction of aptamer-thrombin via an arrested rolling circle amplification to yield horseradish peroxidase (HRP)-mimicking DNAzymes as biocatalysts.
一种通过过氧化氢还原金离子实现金纳米颗粒生长的灵敏信号生成机制被应用于等离子体适体传感器中，基于适体与凝血酶的特异性相互作用，通过停滞滚环扩增产生模仿辣根过氧化物酶（HRP）的脱氧核酶作为生物催化剂，从而实现单分子水平的凝血酶裸眼检测。
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