Fujioka Yoichiro, Nanbo Asuka, Nishide Shin-Ya, Ohba Yusuke
Department of Cell Physiology, Hokkaido University Graduate School of Medicine.
Anal Sci. 2015;31(4):267-74. doi: 10.2116/analsci.31.267.
In response to extracellular stimuli, cells display a variety of behaviors, including proliferation, differentiation, morphological changes and migration. The analysis of the spatiotemporal regulation of signal transduction in living cells is needed for a better understanding of such behaviors, and such investigations have been greatly accelerated by the development of fluorescent protein-based biosensors. Currently, by using these biosensors a range of molecular actions, including lipid metabolism, protein activation, and ion dynamics, can be visualized in living cells. We recently reported that intracellular calcium, with its relevant downstream signaling pathways consisting of the small GTPase Ras and the lipid kinase phoshoinositide-3-kinase (PI3K), can be exploited in an efficient incorporation of influenza A viruses into host cells via endocytosis using a set of biosensors based on fluorescent proteins and the principle of Förster resonance energy transfer. Here, we focus this review on fluorescent protein-based biosensors that have been utilized in our recent research reports.
作为对细胞外刺激的反应，细胞会表现出多种行为，包括增殖、分化、形态变化和迁移。为了更好地理解这些行为，需要对活细胞中信号转导的时空调节进行分析，而基于荧光蛋白的生物传感器的发展极大地加速了此类研究。目前，通过使用这些生物传感器，可以在活细胞中观察到一系列分子作用，包括脂质代谢、蛋白质激活和离子动态变化。我们最近报道，利用一组基于荧光蛋白和福斯特共振能量转移原理的生物传感器，细胞内钙及其由小GTP酶Ras和脂质激酶磷酸肌醇-3-激酶（PI3K）组成的相关下游信号通路，可以通过内吞作用有效地将甲型流感病毒纳入宿主细胞。在此，我们将这篇综述的重点放在我们最近的研究报告中所使用的基于荧光蛋白的生物传感器上。
