[电针对颈椎病大鼠椎间盘纤维环细胞中Wnt-β-连环蛋白信号通路的影响]
[Effects of electroacupuncture on Wnt-β-catenin signal pathway in annulus fibrosus cells in intervertebral disc in rats with cervical spondylosis].
作者信息
Liao Jun, Xie Qiao-Yu, Zhang Le, Ke Mei-Gui
出版信息
Zhongguo Zhen Jiu. 2014 Dec;34(12):1203-7.
OBJECTIVE
To observe the effects of electroacupuncture (EA) at "Dazhui" (GV 14) on Wnt-β-catenin signal pathway in annulus fibrosus cells in intervertebral disc in rats with cervical spondylosis.
METHODS
Forty SD rats were randomized into a control group, a model group, an EA group and a medication group, 10 rats in each one. Rats in the control group were treated with sham operation, only incision on local skin; rats in the remaining groups were made into cervical spondylosis models. After model establishment, rats in the control group and model group received fixed treatment under identical condition; rats in the EA group were treated with EA at "Dazhui" (GV 14), 30 min per treatment; rats in the medication group were treated with intragastric administration of meloxicam tablets. Treatments were both given once a day, and 14 days were taken as one session; there was an interval of 2 days between two sessions, and totally two sessions were given. After the treatments, immunohistochemistry was applied to measure the expression of Wnt, glycogen synthase kinase-3β (GSK-3β) and Axin in annulus fibrosus cells; western blot was used to test the expression of P-β-catenin.
RESULTS
In the control group, there were more positive cells of Wnt, GSK-3β and Axin, which were intensively distributed, deeply colored, and strongly positive; In the model group, there were less positive cells of Wnt, GSK-3β and Axin, which were sparsely distributed and weakly positive. The expression of Wnt, GSK-3β, Axin and P-β-catenin in the model group was less than that in the control group (all P < 0.05); expression of Wnt, GSK-3β, Axin and P-β-catenin in the EA group and medication group was higher than that in the model group (all P < 0.05); expression of Wnt, GSK-3β, Axin and P-β-catenin was not significantly different between EA group and medication group (all P > 0.05).
CONCLUSION
EA could delay the degeneration of intervertebral disc, which may be related to EA inhibiting signal pathway of Wnt-β-catenin.
目的
观察电针“大椎”（GV 14）对颈椎病大鼠椎间盘纤维环细胞中Wnt-β-连环蛋白信号通路的影响。
方法
将40只SD大鼠随机分为对照组、模型组、电针组和药物组，每组10只。对照组大鼠行假手术，仅切开局部皮肤；其余各组大鼠制作颈椎病模型。造模成功后，对照组和模型组大鼠在相同条件下进行固定处理；电针组大鼠采用电针“大椎”（GV 14）治疗，每次治疗30分钟；药物组大鼠灌胃给予美洛昔康片。治疗均每日1次，14天为1个疗程；两个疗程之间间隔2天，共给予两个疗程。治疗后，采用免疫组织化学法检测纤维环细胞中Wnt、糖原合酶激酶-3β（GSK-3β）和Axin的表达；采用蛋白质印迹法检测磷酸化β-连环蛋白（P-β-catenin）的表达。
结果
对照组Wnt、GSK-3β和Axin阳性细胞较多，分布密集，染色深，呈强阳性；模型组Wnt、GSK-3β和Axin阳性细胞较少，分布稀疏，呈弱阳性。模型组Wnt, GSK-3β, Axin和P-β-catenin的表达低于对照组（均P < 0.05）；电针组和药物组Wnt, GSK-3β, Axin和P-β-catenin的表达高于模型组（均P < 0.05）；电针组和药物组Wnt, GSK-3β, Axin和P-β-catenin的表达差异无统计学意义（均P > 0.05）。
结论
电针可延缓椎间盘退变，其机制可能与抑制Wnt-β-连环蛋白信号通路有关。
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