Minuth Will W, Denk Lucia
Molecular and Cellular Anatomy, University of Regensburg, University Street 31, 93053, Regensburg, Germany.
Cytotechnology. 2016 Mar;68(2):179-96. doi: 10.1007/s10616-015-9873-x. Epub 2015 Apr 17.
To meet specific requirements of developing tissues urgently needed in tissue engineering, biomaterial research and drug toxicity testing, a versatile perfusion culture system was developed. First an individual biomaterial is selected and then mounted in a MINUSHEET(®) tissue carrier. After sterilization the assembly is transferred by fine forceps to a 24 well culture plate for seeding cells or mounting tissue on it. To support spatial (3D) development a carrier can be placed in various types of perfusion culture containers. In the basic version a constant flow of culture medium provides contained tissue with always fresh nutrition and respiratory gas. For example, epithelia can be transferred to a gradient container, where they are exposed to different fluids at the luminal and basal side. To observe development of tissue under the microscope, in a different type of container a transparent lid and base are integrated. Finally, stem/progenitor cells are incubated in a container filled by an artificial interstitium to support spatial development. In the past years the described system was applied in numerous own and external investigations. To present an actual overview of resulting experimental data, the present paper was written.
为满足组织工程、生物材料研究和药物毒性测试中对急需发育组织的特定要求，开发了一种多功能灌注培养系统。首先选择单个生物材料，然后将其安装在MINUSHEET(®)组织载体中。灭菌后，用精细镊子将组件转移到24孔培养板中用于接种细胞或在其上安装组织。为支持空间（3D）发育，载体可放置在各种类型的灌注培养容器中。在基本版本中，恒定的培养基流为所含组织提供始终新鲜的营养和呼吸气体。例如，上皮组织可转移到梯度容器中，在那里它们在管腔侧和基底侧暴露于不同的流体。为了在显微镜下观察组织的发育，在另一种类型的容器中集成了透明的盖子和底部。最后，将干/祖细胞在充满人工间质的容器中孵育以支持空间发育。在过去几年中，所述系统已应用于众多自身和外部研究中。为了展示所得实验数据的实际概况，撰写了本论文。
