Iakovoglou Valasia, Radoglou Kalliopi
J Environ Biol. 2015 Mar;36(2):345-9.
Biodiversity levels could be enhanced when regenerating a site by seed-derived seedlings. However, seed dormancy poses limitations for many species. As a result, nurseries either produce seedlings from species where dormancy is not an obstacle, or they propagate through cuttings with the risk of decreasing the genetic diversity within and among species at the regenerated sites. In the present study, breaking of seed dormancy was investigated in valuable Mediterranean species of Prunus avium, Prunus spinosa and Rosa canina Specifically, in order to break dormancy, seeds of those species were warm-, cold-stratified and chemically treated. Based on the results, maximum germination for P. avium was 12% when seeds were warm stratified for four weeks altered with eight weeks of cold stratification. For P. spinosa, maximum percent germination was 26% when seeds were warm stratified for two weeks and continuously altered for eight weeks of cold stratification. Finally, for R. canina maximum percent germination was 40% under four weeks of warm stratification altered with twenty weeks of cold stratification, when seeds were pretreated with H2SO4 for 15 min. A maximum of twelve weeks of cold stratification for P. avium, P. spinosa and 20 weeks for R. canina provided almost zero percent germination. The results indicated that all three species experienced intense dormancy levels suggesting that those species need to be treated properly prior to sowing. Nonetheless, additional experiments are needed to achieve greater germination percentage of highly valuable species in orderto encourage seed derived seedling production.
通过种子萌发的幼苗对场地进行再生时，生物多样性水平可能会提高。然而，种子休眠对许多物种构成了限制。因此，苗圃要么培育那些休眠不是障碍的物种的幼苗，要么通过扦插繁殖，但这样做有降低再生场地内物种间和物种内遗传多样性的风险。在本研究中，对地中海地区有价值的樱桃李、黑刺李和犬蔷薇等物种的种子休眠破除进行了研究。具体而言，为了破除休眠，对这些物种的种子进行了暖层积、冷层积和化学处理。根据结果，樱桃李种子在暖层积四周后再冷层积八周时，最大发芽率为12%。对于黑刺李，种子暖层积两周并持续冷层积八周时，最大发芽率为26%。最后，对于犬蔷薇，种子用硫酸预处理15分钟后，暖层积四周并冷层积二十周时，最大发芽率为40%。樱桃李和黑刺李最多进行十二周的冷层积，犬蔷薇进行二十周的冷层积时，发芽率几乎为零。结果表明，这三个物种都经历了强烈的休眠水平，这表明这些物种在播种前需要进行适当处理。尽管如此，还需要进行更多实验，以提高高价值物种的发芽率，从而促进种子繁殖幼苗的生产。
