TCF7L2基因rs7903146（C/T）多态性对喀麦隆2型糖尿病易感性的影响
Contribution of the TCF7L2 rs7903146 (C/T) gene polymorphism to the susceptibility to type 2 diabetes mellitus in Cameroon.
作者信息
Guewo-Fokeng Magellan, Sobngwi Eugene, Atogho-Tiedeu Barbara, Donfack Olivier Sontsa, Noubiap Jean Jacques N, Ngwa Elvis Ndonwi, Mato-Mofo Edith Pascale, Fosso Priscille Pokam, Djahmeni Eric, Djokam-Dadjeu Rosine, Evehe Marie-Solange, Aminkeng Folefac, Mbacham Wilfred F, Mbanya Jean Claude
机构信息
Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon ; Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon ; Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon ; National Obesity Center, Yaoundé Central Hospital, University of Yaoundé 1, Yaoundé, Cameroon.
出版信息
J Diabetes Metab Disord. 2015 Apr 14;14:26. doi: 10.1186/s40200-015-0148-z. eCollection 2015.
BACKGROUND
Data on the genetic variants for type 2 diabetes mellitus (T2DM) in sub-Saharan African populations are very scarce. This study aimed to investigate the association of transcription factor 7-like (TCF7L2) with T2DM in a Cameroonian population and explore possible genotype-phenotype correlation.
METHODS
This is a case-control study involving 37 T2DM patients and 37 non-diabetic volunteers of Cameroonian ethnicity aged 40 years old and above. We collected clinical and biological data to determine phenotypic traits. TCF7L2 was analyzed by genotyping for rs7903146 (C/T) using PCR-RFLP. Biochemical analyses were performed using a spectrophotometer with Chronolab kits. Statistical analyses were carried out using IBM SPSS, PS and Quanto.
RESULTS
TCF7L2 was associated with T2DM in this Cameroonian population (p = 0.013 for alleles, and p = 0.013 for genotypes). The risk allele was C (9.5% patients vs. 0% healthy controls, OR = 16.56) and the protective allele was T (90.5% patients vs. 100.0% healthy controls, OR = 0.06). The risk genotype was C/T (18.9% patients vs. 0% healthy controls, OR = 18.44), while the protective genotype was T/T (81.1% patients vs. 100.0% healthy controls, OR = 0.054). The statistical power was 99.99%. TCF7L2 was not preferentially associated with a specific disease phenotype.
CONCLUSION
TCF7L2 is associated with T2DM in this Cameroonian population. The association is not dependent on a specific T2DM phenotype. Clinical genetic testing for TCF7L2 can help to predict the occurrence of T2DM in Cameroon.
背景
撒哈拉以南非洲人群中2型糖尿病（T2DM）基因变异的数据非常稀少。本研究旨在调查喀麦隆人群中转录因子7样蛋白（TCF7L2）与T2DM的关联，并探索可能的基因型-表型相关性。
方法
这是一项病例对照研究，纳入了37例40岁及以上的喀麦隆族T2DM患者和37名非糖尿病志愿者。我们收集了临床和生物学数据以确定表型特征。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对rs7903146（C/T）进行基因分型来分析TCF7L2。使用配备Chronolab试剂盒的分光光度计进行生化分析。使用IBM SPSS、PS和Quanto进行统计分析。
结果
在该喀麦隆人群中，TCF7L2与T2DM相关（等位基因p = 0.013，基因型p = 0.013）。风险等位基因为C（患者中占9.5%，健康对照中占0%，比值比[OR]=16.56），保护等位基因为T（患者中占90.5%，健康对照中占100.0%，OR = 0.06）。风险基因型为C/T（患者中占18.9%，健康对照中占0%，OR = 18.44），而保护基因型为T/T（患者中占81.1%，健康对照中占100.0%，OR = 0.054）。统计效能为99.99%。TCF7L2与特定疾病表型无优先关联。
结论
在该喀麦隆人群中，TCF7L2与T2DM相关。这种关联不依赖于特定的T2DM表型。对TCF7L2进行临床基因检测有助于预测喀麦隆T2DM的发生。
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