Kinugasa H, Nouso K, Tanaka T, Miyahara K, Morimoto Y, Dohi C, Matsubara T, Okada H, Yamamoto K
Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
1] Department of Gastroenterology and Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan [2] Department of Molecular Hepatology, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
Br J Cancer. 2015 May 12;112(10):1652-5. doi: 10.1038/bjc.2015.129. Epub 2015 Apr 21.
Although there are some new criteria for human epidermal growth factor receptor 2 (HER2) expression with immunohistochemistry/fluorescence in situ hybridisation (IHC/FISH) in gastric cancer, the method is still ambiguous and is somewhat dependent on the subjective qualities of the evaluator.
We used droplet digital polymerase chain reaction (ddPCR) to evaluate HER2 amplification in formalin-fixed and paraffin-embedded (FFPE) samples and cell-free serum circulating tumour DNA (ctDNA) in 25 patients with gastric cancer.
The concordance rate of HER2 amplification examined in FFPE samples with ddPCR and IHC/FISH was 92% (23 out of 25). The concordance rate of FFPE with ctDNA was not high (62.5%); however, patients who were HER2-positive by ctDNA had significantly shorter survival compared with HER2-negative patients.
Our results demonstrated that this ddPCR method was as effective as IHC/FISH and therefore might become a standard method for analysing not only FFPE but also ctDNA.
尽管在胃癌中，通过免疫组织化学/荧光原位杂交（IHC/FISH）检测人表皮生长因子受体2（HER2）表达有一些新的标准，但该方法仍不明确，且在一定程度上依赖于评估者的主观素质。
我们使用液滴数字聚合酶链反应（ddPCR）来评估25例胃癌患者福尔马林固定石蜡包埋（FFPE）样本中的HER2扩增以及游离血清循环肿瘤DNA（ctDNA）。
通过ddPCR检测FFPE样本中HER2扩增与IHC/FISH的一致性率为92%（25例中的23例）。FFPE与ctDNA的一致性率不高（62.5%）；然而，ctDNA检测为HER2阳性的患者与HER2阴性患者相比，生存期明显更短。
我们的结果表明，这种ddPCR方法与IHC/FISH一样有效，因此不仅可能成为分析FFPE样本，也可能成为分析ctDNA的标准方法。
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