[感染约氏疟原虫的小鼠在感染早期Treg/Thl7细胞的比例]
[The ratio of Treg/Thl7 cells from mice infected with Plasmodium yoelii in the early stage of infection].
作者信息
Chen Guang, Liu Lei, Bi Sheng, Luo Lan, Wang Fang-fang, Cai Lian-shun, Su Ju-xiang, Dai Yue
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2014 Dec;32(6):425-9.
OBJECTIVE
To investigate the change of Treg/Th17 cell ratio in mice infected with Plasmodium yoelii 17XL in the early stage of infection.
METHODS
Forty 6-8 week-old female BALB/c mice were randomly divided into infection group and Treg blockade group. All the mice were infected by intraperitoneal injection with 1 x 10(6) P. yoelii 17XL-parasitized erythrocytes. Mice in Treg blockade group were intraperitoneally injected with 1 mg CD25 mAb before infection and on day 1 post-infection, respectively. Three healthy mice served as control. Tail vein blood samples were collected in two groups each with 10 mice during day 1-7 post-infection. The parasitemia was monitored daily by examining Giemsa-stained thin film. The survival rate of infected mice was calculated. Before infection, on day 3 and 5 post-infection, the spleen was aseptically removed from each mouse in infection group (n=3) and Treg blockade group (n=3), and splenocyte suspensions were prepared. The proportion of Treg and Th17 cells were analyzed by flow cytometry. The ratio of Treg/Th17 cells was calculated and the relative analysis of the percentage of Treg and Th17 cells was conducted.
RESULTS
Parasitemia appeared on day 2 post-infection in infection group. On day 5, the infection rate of P. yoelii in erythrocytes increased to (49.8±4.7)% with a survival rate of 60.0%. All mice died on day 6 post-infection. In Treg blockade group, P. yoelii 17XL-parasitized erythrocytes appeared on day 2 post-infection; the parasitemia was (10.0±3.5)% on day 5, and increased rapidly to 60.0% on day 7; all mice died on day 8. The percentages of Treg and Th17 cells reached (11.1±1.3)% and (16.7±3.3)% respectively on day 3 post-infection in infection group, and increased to (26.1±4.5)% and (19.6±2.6)% on day 5, considerably higher than that of the control (P<0.01). The ratio of Treg/Th17 cells was 0.7±0.1, 1.3±0.4 on day 3 and 5 post-infection in infection group, respectively, which was much lower than that of normal control (2.1±0.5) (P<0.01, P<0.05). On day 3 and 5 post-infection, the percentages of Treg cells in CD4+ T cells was (2.4±1.3)% and (4.3±2.9)%, respectively, and that of Th17 cells reached (26.1±2.2)% and (6.5±2.1)% in Treg blockade group. The ratio of Treg/Th17 cells in Treg blockade group was 0.1±0.1 and 0.7±0.4, respectively, which was considerably lower than that of the control (3.6±0.4)(P<0.01). The proportion of Treg cells in CD4+ T cells was positively correlated with that of Th17 cells (r=0.8166, P<0.01).
CONCLUSION
The ratio of Treg/Th17 cells significantly decreases in the early stage of P. yoelii 17XL infection.
目的
研究约氏疟原虫17XL感染小鼠早期Treg/Th17细胞比值的变化。
方法
将40只6-8周龄雌性BALB/c小鼠随机分为感染组和Treg阻断组。所有小鼠均经腹腔注射1×10(6)个感染约氏疟原虫17XL的红细胞进行感染。Treg阻断组小鼠在感染前及感染后第1天分别经腹腔注射1mg CD25单克隆抗体。3只健康小鼠作为对照。在感染后第1-7天，两组各取10只小鼠采集尾静脉血样本。每日通过吉姆萨染色薄血膜检查监测疟原虫血症。计算感染小鼠的存活率。在感染前、感染后第3天和第5天，从感染组（n=3）和Treg阻断组（n=3）的每只小鼠无菌取出脾脏，制备脾细胞悬液。通过流式细胞术分析Treg和Th17细胞的比例。计算Treg/Th17细胞比值，并对Treg和Th17细胞百分比进行相关性分析。
结果
感染组在感染后第2天出现疟原虫血症。第5天，红细胞内约氏疟原虫感染率升至(49.8±4.7)%，存活率为60.0%。所有小鼠在感染后第6天死亡。在Treg阻断组，感染后第2天出现感染约氏疟原虫17XL的红细胞；第5天疟原虫血症为(10.0±3.5)%，第7天迅速升至60.0%；所有小鼠在感染后第8天死亡。感染组在感染后第3天Treg和Th17细胞百分比分别达到(11.1±1.3)%和(16.7±3.3)%，第5天升至(26.1±4.5)%和(19.6±2.6)%，显著高于对照组（P<0.01）。感染组在感染后第3天和第5天Treg/Th17细胞比值分别为0.7±0.1、1.3±0.4，远低于正常对照组（2.1±0.5）（P<0.01，P<0.05）。在感染后第3天和第5天，Treg阻断组CD4+T细胞中Treg细胞百分比分别为(2.4±1.3)%和(4.3±2.9)%，Th17细胞百分比分别达到(26.1±2.2)%和(6.5±2.1)%。Treg阻断组Treg/Th17细胞比值分别为0.1±0.1和0.7±0.4，显著低于对照组（3.6±0.4）（P<0.01）。CD4+T细胞中Treg细胞比例与Th17细胞比例呈正相关（r=0.8166，P<0.01）。
结论
约氏疟原虫17XL感染早期Treg/Th17细胞比值显著降低。
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