High-performance liquid chromatographic determination of tilidine in pharmaceutical dosage forms.

A reversed-phase high performance liquid chromatographic method for the determination of tilidine in bulk drug and its liquid and solid dosage forms is described. The method uses reversed-phase column RP-8 with methanol -0.2% water solution of ammonium carbonate (75:25,v/v) as the mobile phase and UV detector. The time taken for separation is 6.17 min. The response is linear up to 3.6 mmol/l, with recovery levels ranging from 98.2 to 101.2%. The present method is rapid, simple and reproducible.