Guo Jian-Hui, Ma Wei, Yang Jin-Wei, Gao Yan, Liang Zhang, Liu Jia, Wang Dong-Yan, Luo Tao, Cheng Jing-Ru, Li Li-Yan
Second Department of General Surgery, First People's Hospital of Yunnan Province, Kunming, 650032, Yunnan, China,
Childs Nerv Syst. 2015 Jun;31(6):863-72. doi: 10.1007/s00381-015-2713-7. Epub 2015 Apr 23.
The development of the human embryonic spinal cord is very complicated, and many cell types are involved in the process. However, the morphological characteristics of neuronal and glial cells during the development of the human fetal spinal cord have not been described. We investigated the systemic distributions and expression pattern of the cell type-specific markers Neuron-specific nuclear protein (NeuN) and glial fibrillary acidic protein (GFAP) during the development of the human fetal spinal cord, in order to clarify the detailed developmental changes of neuronal and glial cells in chronological and spatial aspects.
A total of 35 fetuses, aged 3 weeks to 8 months of gestation (E3W-E8M), were studied. The markers used for immunohistochemical study were NeuN and GFAP.
The intracellular makers NeuN and GFAP were widely detected expression in different structures and cells during the development of the human fetal spinal cord, including the following: central canal, neuroepithelial layer, internal limiting membrane, mantle layer, marginal layer, basal plate, alar plate, ependymal layer, gray matter, white matter, neuron, astrocytes, and nerve fibers. However, there was an absence of GFAP in astrocytes during early fetal spinal cord development until E9W, and the appearance of GFAP-positive reactivity was later than that of neurons.
We consider that NeuN and GFAP can be used to identify neuronal and glial cells during the development of the human fetal spinal cord, and their distribution differs both chronologically and spatially. These characteristic expression patterns would give us a clue to better understand the developmental characteristics of the human spinal cord.
人类胚胎脊髓的发育非常复杂，该过程涉及多种细胞类型。然而，人类胎儿脊髓发育过程中神经元和神经胶质细胞的形态特征尚未见描述。我们研究了人类胎儿脊髓发育过程中细胞类型特异性标志物神经元特异性核蛋白（NeuN）和胶质纤维酸性蛋白（GFAP）的系统分布及表达模式，以阐明神经元和神经胶质细胞在时间和空间方面的详细发育变化。
共研究了35例妊娠3周龄至8月龄（E3W - E8M）的胎儿。用于免疫组织化学研究的标志物为NeuN和GFAP。
在人类胎儿脊髓发育过程中，细胞内标志物NeuN和GFAP在不同结构和细胞中广泛检测到表达，包括中央管、神经上皮层、内界膜、套层、边缘层、基板、翼板、室管膜层、灰质、白质、神经元、星形胶质细胞和神经纤维。然而，在胎儿脊髓发育早期直到E9W，星形胶质细胞中不存在GFAP，且GFAP阳性反应的出现晚于神经元。
我们认为NeuN和GFAP可用于鉴定人类胎儿脊髓发育过程中的神经元和神经胶质细胞，它们的分布在时间和空间上均有所不同。这些特征性表达模式将为我们更好地理解人类脊髓的发育特征提供线索。
