基于梯度折射率微透镜的光纤光阱的研制及其主成分分析表征
Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis.
作者信息
Nylk J, Kristensen M V G, Mazilu M, Thayil A K, Mitchell C A, Campbell E C, Powis S J, Gunn-Moore F J, Dholakia K
机构信息
SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK ; School of Biology, University of St Andrews, St Andrews, KY16 9TF, UK.
SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK.
出版信息
Biomed Opt Express. 2015 Mar 27;6(4):1512-9. doi: 10.1364/BOE.6.001512. eCollection 2015 Apr 1.
We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200μm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.
我们展示了一种基于高数值孔径渐变折射率（GRIN）微物镜的小型化单光束光纤光学捕获探针。这使得能够在距探针尖端200μm的距离处进行光学捕获。通过功率谱密度分析和基于主成分分析的原始方法对光纤捕获探针进行了实验表征，以实现精确的粒子跟踪。通过光学介导的免疫突触形成展示了其在生物医学显微镜中的应用。
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