Skowronek R, Tomsia M, Droździok K, Kabiesz J
Rafał Skowronek, Department of Forensic Medicine and Forensic Toxicology, Medical University of Silesia in Katowice, Medyków 18, 40-752 Katowice, Poland,e-mail:
Arch Med Sadowej Kryminol. 2014;64(4):254-67. doi: 10.5114/amsik.2014.50530.
In some criminal cases, the use of classical sources of human genetic material is difficult or even impossible. One solution may be the use of insects, especially blowfly larvae which feed on corpses. A recent review of case reports and experimental studies available in biomedical databases has shown that insects can be a valuable source of human mitochondrial and genomic deoxyribonucleic acid (DNA), allowing for an effective analysis of hypervariable region (HVR) sequences and short tandem repeat (STR) profiles, respectively. The optimal source of human DNA is the crop (a part of the gut) of active third-instar blowfly larvae. Pupae and insect faeces can be also used in forensic genetic practice instead of the contents of the alimentary tract.
在一些刑事案件中,使用传统的人类遗传物质来源存在困难甚至是不可能的。一种解决办法可能是使用昆虫,特别是以尸体为食的蝇蛆。最近对生物医学数据库中现有病例报告和实验研究的综述表明,昆虫可以成为人类线粒体和基因组脱氧核糖核酸(DNA)的宝贵来源,分别有助于对高变区(HVR)序列和短串联重复序列(STR)图谱进行有效分析。人类DNA的最佳来源是活跃的三龄蝇蛆的嗉囊(肠道的一部分)。蛹和昆虫粪便也可用于法医遗传学实践,以替代消化道内容物。