Jaffe D R, Bowden G T
Radiation Oncology Department, University Medical School, Tucson, AZ 85724.
Carcinogenesis. 1989 Dec;10(12):2243-7. doi: 10.1093/carcin/10.12.2243.
DNAs from mouse skin tumors (papillomas, squamous cell carcinomas, basal cell carcinomas and pilomatrixomas) initiated with X-irradiation and promoted with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) demonstrated dominant transforming activity by the production of transformed foci in the mouse recipient line, NIH3T3. Dominant transforming activity was not found in DNA isolated from normal mouse epidermis or from the corresponding liver. The NIH3T3 transformants induced with squamous cell carcinoma DNA grew in soft agar and formed tumors in nude mice. Southern blot analysis of primary NIH3T3 transformant DNAs carrying oncogenes from radiation-initiated squamous cell carcinomas indicated that the oncogenes responsible for the transformation of the recipient cells were not Ha-ras, Ki-ras or N-ras genes, nor were they erbB, B-lym, met, neu or raf. The data presented indicate that DNAs from radiation-initiated mouse skin tumors contain dominant transforming genes that are detectable by DNA-mediated gene transfer. The oncogene sequences activated in these radiation-initiated tumors are distinct non-ras transforming genes.
用X射线引发并用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）促发的小鼠皮肤肿瘤（乳头状瘤、鳞状细胞癌、基底细胞癌和毛母质瘤）的DNA，通过在小鼠受体细胞系NIH3T3中产生转化灶而表现出显性转化活性。从正常小鼠表皮或相应肝脏分离的DNA中未发现显性转化活性。用鳞状细胞癌DNA诱导的NIH3T3转化细胞在软琼脂中生长并在裸鼠中形成肿瘤。对携带来自辐射引发的鳞状细胞癌的癌基因的原代NIH3T3转化细胞DNA进行Southern印迹分析表明，负责受体细胞转化的癌基因不是Ha - ras、Ki - ras或N - ras基因，也不是erbB、B - lym、met、neu或raf。所呈现的数据表明，来自辐射引发的小鼠皮肤肿瘤的DNA含有可通过DNA介导的基因转移检测到的显性转化基因。在这些辐射引发的肿瘤中激活的癌基因序列是不同非ras转化基因。
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