绘制乳腺癌细胞上的 CXCR4 受体图谱。
Mapping the CXCR4 receptor on breast cancer cells.
机构信息
Department of Biomedical Engineering, The City College of New York, 160 Convent Avenue, New York, NY 10031, United States.
Department of Biomedical Engineering, The City College of New York, 160 Convent Avenue, New York, NY 10031, United States; Vascular Biology Program, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, United States; Department of Surgery, Harvard Medical School and Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, United States.
出版信息
Biomaterials. 2015 Jul;57:161-8. doi: 10.1016/j.biomaterials.2015.04.023. Epub 2015 Apr 28.
The CXCR4 receptor triggers cell migration and, in breast cancer, promotes metastasis. To date, the dynamic assembly of CXCR4 on the cell surface as a mediator of receptor binding is not well characterized. The objective of this work is to quantify the density, spatial organization, and magnitude of binding of the CXCR4 receptor on live metastatic breast cancer (MBC) cells. We measured the Young's modulus, the CXCR4 surface density, and CXCR4 unbinding force on MBC cells by atomic force microscopy. We conclude that the CXCR4 density, spatial organization, and matrix stiffness are paramount to achieve strong binding.
趋化因子受体 4（CXCR4）触发细胞迁移，在乳腺癌中促进转移。迄今为止，作为受体结合介体的细胞表面上 CXCR4 的动态组装尚未得到很好的描述。这项工作的目的是量化活转移性乳腺癌 (MBC) 细胞上 CXCR4 受体的密度、空间组织和结合强度。我们通过原子力显微镜测量了 MBC 细胞的杨氏模量、CXCR4 表面密度和 CXCR4 解吸力。我们得出的结论是，CXCR4 的密度、空间组织和基质硬度对于实现强结合至关重要。
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