Mata-Gómez Marco, Mussatto Solange I, Rodríguez Raul, Teixeira Jose A, Martinez Jose L, Hernandez Ayerim, Aguilar Cristóbal N
Department of Food Research (DIA-UAdeC). School of Chemistry, Universidad Autónoma de Coahuila, Saltillo, 25000, Coahuila, Mexico.
Appl Biochem Biotechnol. 2015 Jun;176(4):1131-40. doi: 10.1007/s12010-015-1634-y. Epub 2015 Apr 29.
Gallic acid production in a batch bioreactor was evaluated using as catalytic material the mouldy polyurethane solids (MPS) obtained from a solid-state fermentation (SSF) bioprocess carried out for tannase production by Aspergillus niger GH1 on polyurethane foam powder (PUF) with 5 % (v/w) of tannic acid as inducer. Fungal biomass, tannic acid consumption and tannase production were kinetically monitored. SSF was stopped when tannase activity reached its maximum level. Effects of washing with distilled water and drying on the tannase activity of MPS were determined. Better results were obtained with dried and washed MPS retaining 84 % of the tannase activity. Maximum tannase activity produced through SSF after 24 h of incubation was equivalent to 130 U/gS with a specific activity of 36 U/mg. The methylgallate was hydrolysed (45 %) in an easy, cheap and fast bioprocess (30 min). Kinetic parameters of tannase self-immobilized on polyurethane particles were calculated to be 5 mM and 04.1 × 10(-2) mM/min for K M and V max, respectively. Results demonstrated that the MPS, with tannase activity, can be successfully used for the production of the antioxidant gallic acid from methyl-gallate substrate. Direct use of PMS to produce gallic acid can be advantageous as no previous extraction of enzyme is required, thus reducing production costs.
使用从黑曲霉GH1在含5%（v/w）单宁酸作为诱导剂的聚氨酯泡沫粉末（PUF）上进行固态发酵（SSF）生物过程获得的发霉聚氨酯固体（MPS）作为催化材料，评估分批生物反应器中没食子酸的生产。对真菌生物量、单宁酸消耗和单宁酶生产进行了动力学监测。当单宁酶活性达到最高水平时停止固态发酵。测定了用蒸馏水洗涤和干燥对MPS单宁酶活性的影响。干燥和洗涤后的MPS保留了84%的单宁酶活性，效果更好。培养24小时后通过固态发酵产生的最大单宁酶活性相当于130 U/gS，比活性为36 U/mg。在一个简便、廉价且快速的生物过程（30分钟）中，没食子酸甲酯被水解（45%）。计算得出固定在聚氨酯颗粒上的单宁酶的动力学参数，K M为5 mM，V max为04.1×10(-2) mM/min。结果表明，具有单宁酶活性的MPS可成功用于从没食子酸甲酯底物生产抗氧化剂没食子酸。直接使用PMS生产没食子酸可能具有优势，因为无需预先提取酶，从而降低了生产成本。
