用巴西胡椒精油（Schinus terebinthifolius Raddi）处理的大鼠的肥大细胞浓度和皮肤伤口收缩情况。
Mast cell concentration and skin wound contraction in rats treated with Brazilian pepper essential oil (Schinus terebinthifolius Raddi).
作者信息
Estevão Lígia Reis Moura, Medeiros Juliana Pinto de, Simões Ricardo Santos, Arantes Rosa Maria Esteves, Rachid Milene Alvarenga, Silva Regildo Márcio Gonçalves da, Mendonça Fábio de Souza, Evêncio-Neto Joaquim
机构信息
UFRPE, Recife, PE, Brazil.
UNIFESP, Brazil.
出版信息
Acta Cir Bras. 2015 Apr;30(4):289-95. doi: 10.1590/S0102-865020150040000008. Epub 2015 Apr 1.
PURPOSE
To evaluate wound contraction and the concentration of mast cells in skin wounds treated with 5% BPT essential oil-based ointment in rats.
METHODS
Twenty rats, male, of adult age, were submitted to skin surgery on the right (RA) and left antimeres (LA) of the thoracic region. They were divided into two groups: control (RA - wounds receiving daily topical application of vaseline and lanolin) and treated (LA - wounds treated daily with the topical ointment). The skin region with wounds were collected at days 4, 7, 14 and 21 after surgery. Those were fixed in 10% formaldehyde and later processed for paraffin embedding. Sections were obtained and stained by H.E for histopathology analysis. The degree of epithelial contraction was measured and mast cell concentration were also evaluated.
RESULTS
The treated group showed higher mast cell concentrations (p<0.05) associated with increased contraction at day 7 and 14 respectively.
CONCLUSION
Ointment containing 5% Brazilian pepper tree oil increases mast cell concentration and promotes skin wound contraction in rats.
目的
评估5%巴西胡椒树精油软膏对大鼠皮肤伤口的收缩作用及肥大细胞浓度。
方法
选用20只成年雄性大鼠，在其胸部右（RA）侧和左对侧（LA）进行皮肤手术。将大鼠分为两组：对照组（RA - 伤口每日局部涂抹凡士林和羊毛脂）和治疗组（LA - 伤口每日局部涂抹软膏）。术后第4、7、14和21天收集伤口所在皮肤区域。将组织固定于10%甲醛中，随后进行石蜡包埋处理。制备切片并用苏木精-伊红染色进行组织病理学分析。测量上皮收缩程度并评估肥大细胞浓度。
结果
治疗组在第7天和第14天分别显示出较高的肥大细胞浓度（p<0.05），且与收缩增加相关。
结论
含5%巴西胡椒树油的软膏可增加大鼠肥大细胞浓度并促进皮肤伤口收缩。
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