Yang Ui-Jeong, Maeng Hyojin, Park Tae-Sik, Shim Soon-Mi
1 Department of Food Science and Technology, Sejong University , Seoul, Korea.
2 Department of Life Science, Gachon University , Gyeonggi, Korea.
J Med Food. 2015 Sep;18(9):1022-31. doi: 10.1089/jmf.2014.3371. Epub 2015 Apr 29.
Vascular function is mediated by various regulatory molecules, including endothelial nitric oxide (NO), which regulates the vasodilation of smooth muscle cells. We investigated whether standardized Houttuynia cordata extract (SHCE) could improve physical endurance performance by regulating the endothelial production of NO. For the standardization of Houttuynia cordata (HC) extract, its bioactive components were identified and quantified using ultraperformance liquid chromatography-mass spectrometry. Bioaccessibility and biological activity were measured by the in vitro digestion model system and free radical scavenging capacity, respectively. The vascular function in the endothelium was assessed by the phosphorylation of endothelial nitric oxide synthase (eNOS). A preliminary clinical trial was carried out to assess the physical endurance performance. HC extract was standardized to bioactive components, including chlorogenic acid, rutin, and quercitrin, with the concentration of 5.53, 6.09, and 16.15 mg from 1 g of dry weight, respectively. Bioaccessibility was 33.17%, 31.67%, and 11.18% for chlorogenic acid, rutin, and quercitrin, respectively. Antioxidant activities of SHCE were expressed as vitamin C equivalent antioxidant capacity in 55.81 and 17.23 mg/g of HC extract using ABTS and DPPH scavenging assay, respectively. In human aortic endothelial cells, insulin-mediated phosphorylation of eNOS was increased by SHCE in the presence of palmitate. However, the expression of blood pressure-regulating genes was not altered. The level of blood lactate concentration and the heart rate of subjects who drank SHCE were lower than those of subjects who drank plain water. Oxygen uptake from subjects drinking SHCE was slightly higher than that from those who drank plain water. This study demonstrated that SHCE decreased heart rate and blood lactate, increased oxygen uptake, and improved physical performance, presumably due to the increased NO production.
血管功能由多种调节分子介导，包括内皮一氧化氮（NO），它调节平滑肌细胞的血管舒张。我们研究了标准化的鱼腥草提取物（SHCE）是否能通过调节内皮细胞产生NO来提高身体耐力表现。为了使鱼腥草（HC）提取物标准化，使用超高效液相色谱 - 质谱法对其生物活性成分进行了鉴定和定量。分别通过体外消化模型系统和自由基清除能力来测量生物可及性和生物活性。通过内皮一氧化氮合酶（eNOS）的磷酸化来评估内皮中的血管功能。进行了一项初步临床试验以评估身体耐力表现。HC提取物被标准化为含有绿原酸、芦丁和槲皮苷等生物活性成分，每克干重中它们的浓度分别为5.53、6.09和16.15毫克。绿原酸、芦丁和槲皮苷的生物可及性分别为33.17%、31.67%和11.18%。SHCE的抗氧化活性分别使用ABTS和DPPH清除试验以55.81和17.23毫克/克的HC提取物中的维生素C等效抗氧化能力来表示。在人主动脉内皮细胞中，在棕榈酸存在的情况下，SHCE增加了胰岛素介导的eNOS磷酸化。然而，血压调节基因的表达没有改变。饮用SHCE的受试者的血乳酸浓度水平和心率低于饮用白开水的受试者。饮用SHCE的受试者的摄氧量略高于饮用白开水的受试者。这项研究表明，SHCE降低了心率和血乳酸，增加了摄氧量，并改善了身体表现，推测这是由于NO生成增加所致。
