Camossi Lucilene Granuzzio, Fornazari Felipe, Richini-Pereira Virgínia Bodelão, Costa da Silva Rodrigo, Cardia Daniel Fontana Ferreira, Langoni Helio
Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, UNESP Univ Estadual Paulista, Campus de Botucatu, Botucatu, SP, Brazil.
Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, UNESP Univ Estadual Paulista, Campus de Botucatu, Botucatu, SP, Brazil.
Exp Parasitol. 2015 Jul;154:163-9. doi: 10.1016/j.exppara.2015.04.023. Epub 2015 Apr 30.
Toxoplasma gondii is one of the most significant parasite, due its importance in veterinary medicine and in public health, considered a food-borne pathogens, there is no available drug treatments to eliminate it from animal tissue, this reinforce the search for a vaccine against this parasite. This study was aimed to evaluate the dynamic of the distribution of T. gondii in tissues of female Wistar rats and their milk, after the immunization by oral rote with irradiated tachyzoites. One week after pregnancy confirmation, rats was challenged by gavage with T. gondii bradyzoites, oocysts or tachyzoites of T. gondii. Forty-eight pregnant rats were grouped as follows: immunized and challenged with bradyzoites (BZ*); non-immunized and challenged with bradyzoites (BZ); immunized and challenged with oocysts (OC*); non-immunized and challenged with oocysts (OC); immunized and challenged with tachyzoites (TZ*); non-immunized and challenged with tachyzoites (TZ); only immunized (I); control group (C). After parturition, milk samples were collected for 3 weeks and then rats were sacrificed and the tissues and milk samples were researched for T. gondii parasite load determined by the quantitative PCR (qPCR). It was verified that the immunization with irradiated tachyzoites of T. gondii induced the reduction of parasitic load in muscle samples in rats challenged by bradyzoites and oocysts, although not enabled the development of sterile immunity. The detection of parasite DNA in milk was found throughout the lactation period, from immunized and non-immunized rats, however no differences were found in the parasite load caused by immunization.
刚地弓形虫是最重要的寄生虫之一，因其在兽医学和公共卫生中的重要性，被视为食源性病原体，目前尚无有效的药物治疗方法来清除动物组织中的该病原体，这进一步促使人们寻找针对这种寄生虫的疫苗。本研究旨在评估经口给予辐照速殖子免疫后，雌性Wistar大鼠组织及其乳汁中弓形虫的分布动态。确认怀孕一周后，大鼠通过灌胃感染刚地弓形虫的缓殖子、卵囊或速殖子。48只怀孕大鼠分为以下几组：用缓殖子免疫并感染（BZ*）；未免疫并感染缓殖子（BZ）；用卵囊免疫并感染（OC*）；未免疫并感染卵囊（OC）；用速殖子免疫并感染（TZ*）；未免疫并感染速殖子（TZ）；仅免疫（I）；对照组（C）。分娩后，收集3周的乳汁样本，然后处死大鼠，通过定量PCR（qPCR）检测组织和乳汁样本中的弓形虫寄生虫载量。结果证实，用辐照的刚地弓形虫速殖子免疫可降低感染缓殖子和卵囊的大鼠肌肉样本中的寄生虫载量，尽管不能产生无菌免疫。在整个哺乳期，均检测到免疫和未免疫大鼠乳汁中的寄生虫DNA，但免疫对寄生虫载量没有影响。
