多硫酸化肝素内皮抑素对兔碱烧伤诱导的角膜新生血管形成的抑制作用。
Inhibitory effect of polysulfated heparin endostatin on alkali burn induced corneal neovascularization in rabbits.
作者信息
Li Zhao-Na, Yuan Zhong-Fang, Mu Guo-Ying, Hu Ming, Cao Li-Jun, Zhang Ya-Li, Liu Lei, Ge Ming-Xu
机构信息
Department of Ophthalmology, The Second People's Hospital of Jinan, Jinan 250001, Shandong Province, China.
Department of Ophthalmology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong Province, China.
出版信息
Int J Ophthalmol. 2015 Apr 18;8(2):234-8. doi: 10.3980/j.issn.2222-3959.2015.02.04. eCollection 2015.
AIM
To investigate anti-angiogenic effects of polysulfated heparin endostatin (PSH-ES) on alkali burn induced corneal neovascularization (NV) in rabbits.
METHODS
An alkali burn was made on rabbit corneas to induce corneal NV in the right eye of 24 rabbits. One day after burn creation, a 0.2 mL subconjunctival injection of 50 µg/mL PSH-ES, 50 µg/mL recombinant endostatin (ES), or normal saline was administered every other day for a total of 14d (7 injections). Histology and immunohistochemisty were used to examine corneas. Corneal NV growth was evaluated as microvessel quantity and corneal vascular endothelial growth factor (VEGF) expression was measured by immunohistochemical assay.
RESULTS
Subconjunctival injection of ES and PSH-ES resulted in significant corneal NV suppression, but PSH-ES had a more powerful anti-angiogenic effect than ES. Mean VEGF concentration in PSH-ES treated corneas was significantly lower than in ES treated and saline treated corneas. Histological examination showed that corneas treated with either PSH-ES or ES had significantly fewer microvessels than eyes treated with saline. Additionally corneas treated with PSH-ES had significantly fewer microvessels than corneas treated with ES.
CONCLUSION
Both PSH-ES and recombinant ES effectively inhibit corneal NV induced by alkali burn. However, PSH-ES is a more powerful anti-angiogenic agent than ES. This research has the potential to provide a new treatment option for preventing and treating corneal NV.
目的
研究多硫酸化肝素内皮抑素（PSH-ES）对兔碱烧伤诱导的角膜新生血管化（NV）的抗血管生成作用。
方法
对24只兔的右眼进行碱烧伤以诱导角膜NV。烧伤后1天，每隔一天结膜下注射0.2 mL 50μg/mL的PSH-ES、50μg/mL重组内皮抑素（ES）或生理盐水，共14天（7次注射）。采用组织学和免疫组织化学方法检查角膜。通过微血管数量评估角膜NV生长情况，并通过免疫组织化学测定法测量角膜血管内皮生长因子（VEGF）的表达。
结果
结膜下注射ES和PSH-ES均能显著抑制角膜NV，但PSH-ES的抗血管生成作用比ES更强。PSH-ES处理的角膜中VEGF平均浓度显著低于ES处理和生理盐水处理的角膜。组织学检查显示，PSH-ES或ES处理的角膜微血管数量明显少于生理盐水处理的眼睛。此外，PSH-ES处理的角膜微血管数量明显少于ES处理的角膜。
结论
PSH-ES和重组ES均能有效抑制碱烧伤诱导的角膜NV。然而，PSH-ES是一种比ES更强的抗血管生成剂。本研究有可能为预防和治疗角膜NV提供一种新的治疗选择。
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