Rosselli P, Pierattelli M, Ferrari R, Rinaldi R, Rapi G, Mattei R, Noccioli B
Dipartimento di Pediatria, Università di Firenze, Italia.
Pediatr Med Chir. 1989 May-Jun;11(3):301-5.
The aim of this study was to determine the value of IgA and IgG antigliadin antibody test (AGA) for screening, diagnosis and follow-up of coeliac disease. A rapid, simple, sensitive and accurate immunosorbent assay (ELISA) was used to determine AGA IgA and IgG in the sera of patients with untreated coeliac disease (I stage), coeliac patients in gluten withdrawal, healthy controls, children with gastroenterological disorders other than coeliac disease and children with constitutional short stature. In the I stage of coeliac disease the AGA IgA gave a sensitivity of 90.9% and a specificity of 97.9%, the IgG assay resulted in a sensitivity of 100% and a specificity of 82.3%. AGA IgG resulted positive in 17.7% of control disease group, but it's interesting to remark that they had a mean level significantly lower than in coeliac patients. On gluten free diet the titres of AGA IgA returned to normal value in three months, while the AGA IgG showed a slower decrease.
本研究的目的是确定IgA和IgG抗麦胶蛋白抗体检测（AGA）在乳糜泻筛查、诊断及随访中的价值。采用一种快速、简便、灵敏且准确的免疫吸附测定法（ELISA）来测定未经治疗的乳糜泻患者（I期）、戒食麸质的乳糜泻患者、健康对照者、患有非乳糜泻性胃肠疾病的儿童以及体质性身材矮小儿童血清中的AGA IgA和IgG。在乳糜泻I期，AGA IgA的敏感性为90.9%，特异性为97.9%，IgG检测的敏感性为100%，特异性为82.3%。AGA IgG在17.7%的对照疾病组中呈阳性，但值得注意的是，其平均水平显著低于乳糜泻患者。在无麸质饮食情况下，AGA IgA的滴度在三个月内恢复至正常水平，而AGA IgG的下降则较为缓慢。
