肽核酸荧光原位杂交法鉴定水产品和环境中的弧菌属。
Peptide nucleic acid fluorescence in-situ hybridization for identification of Vibrio spp. in aquatic products and environments.
机构信息
Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, People's Republic of China; Food Safety Key Laboratory of Zhejiang Province, Zhejiang Entry-Exit Inspection and Quarantine Bureau, 126 Fuchun Road, Hangzhou 310012, People's Republic of China.
Food Safety Key Laboratory of Zhejiang Province, Zhejiang Entry-Exit Inspection and Quarantine Bureau, 126 Fuchun Road, Hangzhou 310012, People's Republic of China.
出版信息
Int J Food Microbiol. 2015 Aug 3;206:39-44. doi: 10.1016/j.ijfoodmicro.2015.04.017. Epub 2015 Apr 17.
A peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method was developed for specific detection of the Vibrio genus. In silico analysis by BLAST and ProbeCheck showed that the designed PNA probe targeting the 16S rRNAs was suitable for specific identification of Vibrio. Specificity and sensitivity of the probe Vib-16S-1 were experimentally verified by its reactivity against 18 strains of 9 Vibrio species and 14 non-Vibrio strains of 14 representative species. The PNA-FISH assay was able to identify 47 Vibrio positive samples from selectively enriched cultures of 510 samples of aquatic products and environments, comparable with the results obtained by biochemical identification and real-time PCR. We conclude that PNA-FISH can be an alternative method for rapid identification of Vibrio species in a broad spectrum of seafood or related samples.
开发了一种肽核酸荧光原位杂交（PNA-FISH）方法，用于特异性检测弧菌属。BLAST 和 ProbeCheck 的计算机分析表明，针对 16S rRNA 设计的 PNA 探针适合于特异性鉴定弧菌。通过其对 9 种弧菌属的 18 株和 14 种代表性物种的 14 种非弧菌属的反应性，实验验证了探针 Vib-16S-1 的特异性和灵敏度。PNA-FISH 检测法能够从 510 个水产养殖环境样本的选择性富集培养物中鉴定出 47 个弧菌阳性样本，与生化鉴定和实时 PCR 的结果相当。我们得出结论，PNA-FISH 可以作为一种快速鉴定广谱海鲜或相关样本中弧菌属的替代方法。
Zotero 插件