Helmholtz Zentrum München, German Research Center for Environmental Health, Research Unit Environmental Genomics, Ingolstaedter Landstr. 1, 85764 Neuherberg, Germany.
Helmholtz Zentrum München, German Research Center for Environmental Health, Institute for Computational Biology, Ingolstaedter Landstr. 1, 85764 Neuherberg, Germany.
Chemosphere. 2015 Sep;134:307-12. doi: 10.1016/j.chemosphere.2015.04.072. Epub 2015 May 15.
Metformin (MET) as an emerging contaminant has been detected in surface water and wastewater in numerous countries, due to insufficient retention in classical waste water treatment plants. In order to characterize the uptake of the compound during phytotreatment of waste water, a short term Pitman chamber experiment was carried out to assess the characteristics of MET uptake and transport by roots. Three different concentrations (0.5, 1.0 and 2.0 mmol L(-)(1)) were applied to cattail (Typha latifolia) and reed (Phragmites australis) roots which were used to investigate the uptake mechanism because they are frequently utilized in phytoremediation. In addition, quinidine was used as an inhibitor to assess the role of organic cation transporters (OCTs) in the uptake of MET by T. latifolia. The transport process of MET is different from carbamazepine (CBZ) and caffeine (CFN). In both T. latifolia and P. australis, the uptake processes were independent of initial concentrations. Quinidine, a known inhibitor of organic cation transporters, can significantly affect MET uptake by T. latifolia roots with inhibition ratios of 70-74%. Uptake into the root could be characterized by a linear model with R(2) values in the range of 0.881-0.999. Overall, the present study provides evidence that MET is taken up by plant roots and has the potential for subsequent translocation. OCTs could be one of the important pathways for MET uptake into the plant.
二甲双胍（MET）作为一种新兴的污染物，由于在传统的污水处理厂中去除率不高，已在许多国家的地表水和废水中被检出。为了研究植物处理废水过程中对该化合物的吸收，进行了一项短期皮特曼室实验，以评估 MET 被植物根系吸收和运输的特征。三种不同浓度（0.5、1.0 和 2.0 mmol L(-)(1)）被应用于香蒲（Typha latifolia）和芦苇（Phragmites australis）的根系，以研究吸收机制，因为它们经常被用于植物修复。此外，还使用奎尼丁作为抑制剂来评估有机阳离子转运蛋白（OCTs）在香蒲吸收 MET 中的作用。MET 的传输过程与卡马西平（CBZ）和咖啡因（CFN）不同。在香蒲和芦苇中，吸收过程均与初始浓度无关。奎尼丁是有机阳离子转运蛋白的已知抑制剂，可显著影响香蒲根系对 MET 的吸收，抑制率为 70-74%。进入根系的吸收过程可以用线性模型来描述，R(2)值在 0.881-0.999 之间。总体而言，本研究提供了证据表明 MET 被植物根系吸收，并有可能随后被转运。OCTs 可能是 MET 进入植物的重要途径之一。
