School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane QLD-4072, Australia.
Biomacromolecules. 2015 Jun 8;16(6):1807-17. doi: 10.1021/acs.biomac.5b00383. Epub 2015 May 27.
Biopolymers are researched extensively for their applications in biomaterials science and drug delivery including structures and complexes of more than one polymer. Chemical characterization of complexes formed between chitosan (CHI) and alginate dialdehyde (ADA) biopolymers established that while electrostatic interactions dominate (as determined from X-ray photoelectron spectroscopy (XPS)) covalent cross-linking between these biopolymers also contribute to their stability (evidenced from immersion in salt solution). It was furthermore found that imine bond formation could not be directly detected by any of the techniques XPS, FTIR, (1)H NMR, or fluorescence. The layer-by-layer assemblies of the biopolymers formed on silica colloids, glass slides, and alginate hydrogel beads were evaluated using XPS, as well as zeta potential measurements for the silica colloids and changes to hydration properties for the hydrogels. It was found that the degree of oxidation of ADA affected the LbL assemblies in terms of a greater degree of CHI penetration observed when using the more conformationally flexible biopolymer ADA (higher degree of oxidation).
生物聚合物因其在生物材料科学和药物输送中的应用而得到广泛研究，包括多种聚合物的结构和复合物。壳聚糖（CHI）和海藻酸钠二醛（ADA）生物聚合物之间形成的复合物的化学特性研究表明，尽管静电相互作用占主导地位（如 X 射线光电子能谱（XPS）所确定的），但这些生物聚合物之间的共价交联也有助于它们的稳定性（从盐溶液中的浸泡实验中得到证明）。此外，还发现通过任何一种技术（XPS、FTIR、（1）H NMR 或荧光）都无法直接检测到亚胺键的形成。通过 XPS 以及硅溶胶的 ζ 电位测量和水凝胶的水合性质变化，评估了生物聚合物在二氧化硅胶体、玻璃载玻片和海藻酸水凝胶珠上的层层组装。结果发现，ADA 的氧化程度影响 LbL 组装，当使用更具构象灵活性的生物聚合物 ADA（更高的氧化程度）时，观察到 CHI 穿透程度更大。
