[碳酸酐酶IX蛋白在乳腺浸润性导管癌纤维化病灶中的意义]

[Significance of carbonic anhydrase IX protein in fibrotic foci of mammary invasive ductal carcinomas].

作者信息

Li Meiping, Bao Lei, Cai Hongguang, Yang Huiying, Ge Wenshun, Ren Lifang, Lu Bo

机构信息

Department of Pathology, Shaoxing Women and Children Hospital, Shaoxing 312000, China.

Department of Pathology, Shaoxing Women and Children Hospital, Shaoxing 312000, China; E-mail:

出版信息

Zhonghua Bing Li Xue Za Zhi. 2015 Apr;44(4):250-3.

PMID:25975907

Abstract

OBJECTIVE

To study the relationship between fibrotic focus (FF) and carbonic anhydrase (CA) IX in invasive ductal carcinomas (IDC) of the breast.

METHODS

In 167 cases of IDC, the FF was assessed morphologically, and expression of ER, PR and CA IX was evaluated using MaxVision immunohistochemistry.

RESULTS

The expression of CA IX in IDC with and without FF was 56.3% (45/80) and 28.7% (25/87) respectively, with significant difference (P=0.001). In IDC with FF, the CA IX expression of tumor cells in tumors with CA IX-positive fibroblasts (35/40, 87.5%) was significantly (P<0.001) higher than that in tumors with CA IX-negative fibroblasts (10/40, 25.0%). In IDC with FF, the CA IX expression of fibroblasts of FF in grade 3 IDC (23/33, 69.7%) was significantly (P=0.006) higher than that in grade 1+2 tumors (17/47, 36.2%). The ER and PR expression of tumor cells in tumors containing CA IX-negative fibroblasts was 72.5% (29/40) and 65.0% (26/40) respectively, whereas the ER and PR expression of tumor cells in tumors containing CA IX-positive fibroblasts was 50.0% (20/40) and 42.5% (17/40) respectively; the difference was statistically significant (for both ER and PR, P=0.04). The age of patients with tumors containing CA IX-negative fibroblasts was significantly (P=0.002) older than those containing CA IX-positive fibroblasts. The FF diameter/tumor diameter in tumors containing CA IX-positive fibroblasts was significantly larger than those containing CA IX-negative fibroblasts. (3) For the groups of tumor size≤2 cm and tumor size between 2 cm to 5 cm, the diameter of the fibrotic focus was significantly (P<0.01) smaller than the fibrotic focus size of tumors>5 cm in size.

CONCLUSIONS

CA IX expression is correlated with FF, and that in fibroblasts of FF correlated with patients' age, tumor grade, hormone receptors and FF diameter/tumor diameter. CA IX expression in FF might be a marker for poor prognosis in patients with breast cancer.

摘要

目的

研究乳腺浸润性导管癌（IDC）中纤维化灶（FF）与碳酸酐酶（CA）IX之间的关系。

方法

对167例IDC患者，从形态学上评估FF，并采用MaxVision免疫组化法评估雌激素受体（ER）、孕激素受体（PR）和CA IX的表达情况。

结果

有FF的IDC中CA IX的表达率为56.3%（45/80），无FF的IDC中CA IX的表达率为28.7%（25/87），差异有统计学意义（P = 0.001）。在有FF的IDC中，成纤维细胞CA IX阳性的肿瘤中肿瘤细胞的CA IX表达率（35/40，87.5%）显著高于成纤维细胞CA IX阴性的肿瘤（10/40，25.0%）（P < 0.001）。在有FF的IDC中，3级IDC中FF的成纤维细胞CA IX表达率（23/33，69.7%）显著高于1 + 2级肿瘤（17/47，36.2%）（P = 0.006）。成纤维细胞CA IX阴性的肿瘤中肿瘤细胞的ER和PR表达率分别为72.5%（29/40）和65.0%（26/40），而成纤维细胞CA IX阳性的肿瘤中肿瘤细胞的ER和PR表达率分别为50.0%（20/40）和42.5%（17/40）；差异有统计学意义（ER和PR均为P = 0.04）。成纤维细胞CA IX阴性的肿瘤患者年龄显著大于成纤维细胞CA IX阳性的患者（P = 0.002）。成纤维细胞CA IX阳性的肿瘤中FF直径/肿瘤直径显著大于成纤维细胞CA IX阴性的肿瘤。（3）对于肿瘤大小≤2 cm组和肿瘤大小在2 cm至5 cm之间的组，纤维化灶直径显著小于肿瘤大小>5 cm的纤维化灶大小（P < 0.01）。