Institute of Stem Cell Research, Helmholtz Center Munich, Munich, Germany. Ph.D. Program in Biomedicine and Experimental Biology (BEB), Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Institute of Stem Cell Research, Helmholtz Center Munich, Munich, Germany.
Science. 2015 May 15;348(6236):789-93. doi: 10.1126/science.aaa2729.
Adult neural stem cells are the source for restoring injured brain tissue. We used repetitive imaging to follow single stem cells in the intact and injured adult zebrafish telencephalon in vivo and found that neurons are generated by both direct conversions of stem cells into postmitotic neurons and via intermediate progenitors amplifying the neuronal output. We observed an imbalance of direct conversion consuming the stem cells and asymmetric and symmetric self-renewing divisions, leading to depletion of stem cells over time. After brain injury, neuronal progenitors are recruited to the injury site. These progenitors are generated by symmetric divisions that deplete the pool of stem cells, a mode of neurogenesis absent in the intact telencephalon. Our analysis revealed changes in the behavior of stem cells underlying generation of additional neurons during regeneration.
成体神经干细胞是修复受损脑组织的来源。我们使用重复成像技术，在活体完整和受损的成年斑马鱼端脑中追踪单个干细胞，发现神经元是由干细胞直接转化为有丝分裂后神经元和中间祖细胞扩增神经元输出两种方式产生的。我们观察到直接转化消耗干细胞的不平衡和不对称及对称自我更新分裂，导致随着时间的推移干细胞耗竭。脑损伤后，神经元前体细胞被募集到损伤部位。这些前体细胞是通过对称分裂产生的，这种分裂会耗尽干细胞池，这是一种在完整端脑中不存在的神经发生模式。我们的分析揭示了再生过程中产生额外神经元的干细胞行为的变化。
