Sears Patrick R, Yin Wei-Ning, Ostrowski Lawrence E
Cystic Fibrosis/Pulmonary Research and Treatment Center, Marsico Lung Institute, Department of Medicine, University of North Carolina, Chapel Hill, North Carolina.
Cystic Fibrosis/Pulmonary Research and Treatment Center, Marsico Lung Institute, Department of Medicine, University of North Carolina, Chapel Hill, North Carolina
Am J Physiol Lung Cell Mol Physiol. 2015 Jul 15;309(2):L99-108. doi: 10.1152/ajplung.00024.2015. Epub 2015 May 15.
Mucociliary clearance (MCC) is an important innate defense mechanism that continuously removes inhaled pathogens and particulates from the airways. Normal MCC is essential for maintaining a healthy respiratory system, and impaired MCC is a feature of many airway diseases, including both genetic (cystic fibrosis, primary ciliary dyskinesia) and acquired (chronic obstructive pulmonary disease, bronchiectasis) disorders. Research into the fundamental processes controlling MCC, therefore, has direct clinical application, but has been limited in part due to the difficulty of studying this complex multicomponent system in vitro. In this study, we have characterized a novel method that allows human airway epithelial cells to differentiate into a mucociliary epithelium that transports mucus in a continuous circular track. The mucociliary transport device allows the measurement and manipulation of all features of mucociliary transport in a controlled in vitro system. In this initial study, the effect of ciliary beat frequency and mucus concentration on the speed of mucociliary transport was investigated.
黏液纤毛清除（MCC）是一种重要的先天性防御机制，可不断清除气道中吸入的病原体和颗粒物。正常的MCC对于维持健康的呼吸系统至关重要，而MCC受损是许多气道疾病的特征，包括遗传性疾病（囊性纤维化、原发性纤毛运动障碍）和后天性疾病（慢性阻塞性肺疾病、支气管扩张症）。因此，对控制MCC的基本过程的研究具有直接的临床应用价值，但部分由于在体外研究这个复杂的多组分系统存在困难而受到限制。在本研究中，我们描述了一种新方法，该方法可使人气道上皮细胞分化为在连续循环轨道中运输黏液的黏液纤毛上皮。黏液纤毛运输装置允许在可控的体外系统中测量和操纵黏液纤毛运输的所有特征。在这项初步研究中，研究了纤毛摆动频率和黏液浓度对黏液纤毛运输速度的影响。
