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卵胎生硬骨鱼许氏平鲉中低氧诱导因子基因表达及血液学指标对不同氧处理的反应

HIFs genes expression and hematology indices responses to different oxygen treatments in an ovoviviparous teleost species Sebastes schlegelii.

作者信息

Mu Weijie, Wen Haishen, Li Jifang, He Feng

机构信息

Fisheries College, Ocean University of China, Qingdao 266003, China; College of Life Science and Technology, Harbin Normal University, Harbin 150025, China.

Fisheries College, Ocean University of China, Qingdao 266003, China.

出版信息

Mar Environ Res. 2015 Sep;110:142-51. doi: 10.1016/j.marenvres.2015.04.008. Epub 2015 May 8.

Abstract

Hypoxia-inducible factors (HIFs) are transcription factors considered as a respond factor to oxygen tension. By using quantitative real-time PCR, expression files of HIF-1α and HIF-2α mRNA were detected in the Korean rockfish ovary, liver, gill and spleen after 30 min and 60 min acute hypoxia exposure. Meanwhile, the cortisol levels, white blood cells and several serum biochemical values of Korean rockfish under different oxygen concentration treatments were also detected. All the results might be helpful for further understanding of the potential effect of hypoxia in ovoviviparous fish.

摘要

缺氧诱导因子(HIFs)是被认为对氧张力有反应的转录因子。通过定量实时PCR,在急性缺氧暴露30分钟和60分钟后,检测了许氏平鲉卵巢、肝脏、鳃和脾脏中HIF-1α和HIF-2α mRNA的表达谱。同时,还检测了不同氧浓度处理下许氏平鲉的皮质醇水平、白细胞和几种血清生化值。所有这些结果可能有助于进一步了解缺氧对卵胎生鱼类的潜在影响。

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