双黄酮对PC-12细胞中β淀粉样蛋白42细胞毒性的抑制活性。
Inhibitory activities of biflavonoids against amyloid-β peptide 42 cytotoxicity in PC-12 cells.
作者信息
Sasaki Hiroaki, Kitoh Yuki, Tsukada Mariko, Miki Kazuhiko, Koyama Kiyotaka, Juliawaty Lia D, Hakim Euis H, Takahashi Kunio, Kinoshita Kaoru
机构信息
School of Pharmacy, Shujitsu University, Nishigawara 1-6-1, Naka-ku, Okayama-shi, Okayama 703-8516, Japan.
Department of Pharmacognosy and Phytochemistry, Meiji Pharmaceutical University, Noshio 2-522-1, Kiyose-shi, Tokyo 204-8588, Japan.
出版信息
Bioorg Med Chem Lett. 2015 Jul 15;25(14):2831-3. doi: 10.1016/j.bmcl.2015.04.106. Epub 2015 May 11.
A major hallmark of Alzheimer's disease is the cerebral accumulation and resulting cytotoxicity of amyloid-β peptides, particularly Aβ42. In this study, we used an MTT assay to investigate the inhibitory activity of biflavonoids 1-22 against Aβ42 cytotoxicity in PC-12 cell cultures. Cytoprotective effects were observed for the following amentoflavone type biflavonoids: podocarpusflavone B 8, isoginkgetin 10, sciadopitysin 13, and kayaflavone 15. These biflavonoids exhibited strong activity in tested compounds, with EC50 values of 5.18, 10.77, 9.84, and 5.29 μM, respectively. Cell viability tests of PC-12 cells revealed that biflavonoids 13 and 15 had stronger inhibitory activities than apigenin 23 and (-)-epigallocatechin gallate 24.
阿尔茨海默病的一个主要标志是淀粉样β肽，特别是Aβ42在大脑中的积累及其导致的细胞毒性。在本研究中，我们使用MTT法研究了双黄酮1 - 22对PC - 12细胞培养物中Aβ42细胞毒性的抑制活性。观察到以下穗花杉双黄酮型双黄酮具有细胞保护作用：罗汉松黄酮B 8、异银杏双黄酮10、柳杉双黄酮13和 kayaflavone 15。这些双黄酮在受试化合物中表现出较强的活性，EC50值分别为5.18、10.77、9.84和5.29 μM。PC - 12细胞的细胞活力测试表明，双黄酮13和15的抑制活性比芹菜素23和（-）-表没食子儿茶素没食子酸酯24更强。
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