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暴露于铜的尖吻鲈(Lates calcarifer,布洛赫)鳃和肝脏组织的表面超微结构变化

Surface Ultrastructural Changes in the Gill and Liver Tissue of Asian Sea Bass Lates calcarifer (Bloch) Exposed to Copper.

作者信息

Paruruckumani P S, Maharajan A, Ganapiriya V, Narayanaswamy Y, Jeyasekar R Raja

机构信息

PG & Research Department of Zoology, Khadir Mohideen College, Adirampattinam, 614701, Thanjavur Dist, Tamil Nadu, India.

PG & Research Department of Zoology, S.T. Hindu College, Nagercoil, 629002, Kanyakumari Dist, Tamil Nadu, India.

出版信息

Biol Trace Elem Res. 2015 Dec;168(2):500-7. doi: 10.1007/s12011-015-0370-z. Epub 2015 May 26.

Abstract

Surface ultrastructure of the gill and liver of 3-month-old Asian sea bass, Lates calcarifer, after copper exposure, was investigated by scanning electron microscopy (SEM). Fish samples were exposed to copper concentrations of 6.83 and 13.66 ppm (sublethal) for 28 days with parallel untreated control. These structures showed structural modifications in both low and high concentrations of copper exposure. Oedema, hyperplasia, desquamation, necrosis, epithelial lifting, lamellar fusion, collapsed secondary lamellae, curling of secondary lamellae and aneurism in the secondary lamellae were observed in gill tissues exposed to copper. Hepatic lesions related to cloudy swelling of hepatocytes, congestion, vacuolar degeneration, dilation of sinusoids and nuclear hypertrophy were evident in the exposed sea bass liver tissue.

摘要

通过扫描电子显微镜(SEM)研究了3月龄尖吻鲈(Lates calcarifer)在铜暴露后鳃和肝脏的表面超微结构。将鱼样本暴露于浓度为6.83和13.66 ppm(亚致死浓度)的铜中28天,同时设置平行的未处理对照组。在低浓度和高浓度铜暴露下,这些结构均显示出结构改变。在暴露于铜的鳃组织中观察到水肿、增生、脱屑、坏死、上皮隆起、鳃小片融合、次生鳃小片塌陷、次生鳃小片卷曲和次生鳃小片动脉瘤。在暴露的尖吻鲈肝脏组织中,明显出现了与肝细胞混浊肿胀、充血、空泡变性、肝血窦扩张和核肥大相关的肝脏病变。

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