与子痫前期相比，正常妊娠中内皮祖细胞亚群的变化。

Changes in endothelial progenitor cell subsets in normal pregnancy compared with preeclampsia.

作者信息

Parsanezhad Mohammad-Ebrahim, Attar Armin, Namavar-Jahromi Bahia, Khoshkhou Sara, Khosravi-Maharlooei Mohsen, Monabati Ahmad, Habibagahi Mojtaba

机构信息

Department of OB-GYN, Division of Infertility and Reproductive Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Infertility and Reproductive Medicine Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran; Cell and Molecular Medicine Research Club, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Cardiovascular Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

出版信息

J Chin Med Assoc. 2015 Jun;78(6):345-52. doi: 10.1016/j.jcma.2015.03.013. Epub 2015 May 23.

DOI:10.1016/j.jcma.2015.03.013

PMID:26006732

Abstract

BACKGROUND

The results of studies measuring the number of endothelial progenitor cells (EPCs) in normal pregnancies and in preeclampsia have been highly controversial or even contradictory because of cross-sectional designs and different methodologies enumerating three distinct subsets of EPCs: circulating angiogenic cells (CAC), colony-forming unit endothelial cell (CFU-ECs), and endothelial colony forming cells (ECFCs). To provide a clear explanation for these underlying controversies, we designed a prospective study to compare the number of all EPC subsets between three trimesters of normal gestation and a case-control study to compare these values as preeclampsia occurs with those from gestational age (GA) matched normal pregnancy.

METHODS

Samples from peripheral blood of nine women were taken during their three consecutive trimesters of normal pregnancy, and from eight women with preeclampsia. To cover most of the reported phenotypes for CACs and ECFCs in the literature, we enumerated 13 cell populations by quantitative flow cytometry using various combinations of the markers CD34, CD133, CD309, and CD45. We used routine culturing techniques to enumerate CFU-ECs.

RESULTS

The numbers of CACs and ECFCs were higher in women with preeclampsia (p = 0.014). By contrast, preeclampsia was associated with a reduced number of CFU-ECs (p = 0.039). The CAC number rose with the increase in GA (p = 0.016) during normal pregnancy, while the number of CFU-ECs and ECFCs did not differ during the trimesters.

CONCLUSION

Although we did demonstrate an increase in absolute counts of CACs and ECFCs in preeclampsia, fewer colony formation capacities indicated a loss in their functional capabilities. By contrast, the number of CACs increased without alterations in colony formation ability in normal pregnancy with the growth of the fetus. Here, by comparing different methodologies to calculate the number of EPC subsets, we could imitate the existing controversy in the literature for such calculations, which may help to elucidate clearer explanations.

摘要

背景

由于采用横断面设计以及枚举内皮祖细胞（EPC）三个不同亚群（循环血管生成细胞（CAC）、集落形成单位内皮细胞（CFU-EC）和内皮集落形成细胞（ECFC））的方法不同，测量正常妊娠和子痫前期中EPC数量的研究结果一直存在高度争议甚至相互矛盾。为了对这些潜在争议给出清晰解释，我们设计了一项前瞻性研究来比较正常妊娠三个孕期中所有EPC亚群的数量，并设计了一项病例对照研究，以比较子痫前期发生时这些数值与孕周（GA）匹配的正常妊娠的数值。