氯胺酮给药后大鼠的脑代谢组学

Brain metabolomics in rats after administration of ketamine.

作者信息

Wen Congcong, Zhang Meiling, Zhang Yuan, Sun Fa, Ma Jianshe, Hu Lufeng, Lin Guanyang, Wang Xianqin

机构信息

Laboratory Animal Centre of Wenzhou Medical University, Wenzhou, 325035, China.

Analytical and Testing Center of Wenzhou Medical University, Wenzhou, 325035, China.

出版信息

Biomed Chromatogr. 2016 Jan;30(1):81-4. doi: 10.1002/bmc.3518. Epub 2015 Jun 23.

DOI:10.1002/bmc.3518

PMID:26014584

Abstract

In this study, we developed a brain metabolomic method, based on gas chromatography-mass spectrometry (GC/MS), to evaluate the effect of ketamine on rats. Pattern recognition analysis, including both principal component analysis and partial least squares-discriminate analysis revealed that ketamine induced metabolic perturbations. Compared with the control group, the levels of glycerol, uridine, cholesterol in rat brain of the ketamine group (50 mg/kg, 14 days) decreased, while the urea levels increased. Our results indicate that metabolomic methods based on GC/MS may be useful to elucidate ketamine abuse through the exploration of biomarkers.

摘要

在本研究中，我们开发了一种基于气相色谱 - 质谱联用（GC/MS）的脑代谢组学方法，以评估氯胺酮对大鼠的影响。模式识别分析，包括主成分分析和偏最小二乘判别分析，表明氯胺酮会引起代谢紊乱。与对照组相比，氯胺酮组（50 mg/kg，14天）大鼠脑中甘油、尿苷、胆固醇水平降低，而尿素水平升高。我们的结果表明，基于GC/MS的代谢组学方法可能有助于通过探索生物标志物来阐明氯胺酮滥用情况。