[骨代谢相关多种因素对ROS 17/2.8细胞45Ca2+掺入及碱性磷酸酶活性的影响]

[Effects of various factors involved in bone metabolism on 45Ca2+ incorporation and alkaline phosphatase activity of ROS 17/2.8 cells].

作者信息

Kodama A, Shioda Y, Sugita K, Kurihara K, Hosoi K, Ueha T

出版信息

Meikai Daigaku Shigaku Zasshi. 1989;18(1):82-7.

Abstract

The effects of interleukin 1, transforming growth factor-beta (coupling factors), prostaglandin E1, and prostaglandin E2 on incorporation of 45Ca2+ and on alkaline phosphatase activity were studied using cultured ROS 17/2.8 cells, one of cell lines derived from rat osteosarcoma. We found that all these factors stimulate both the incorporation of 45Ca2+ and alkaline phosphatase activity of these cells. On the other hand, one of the bone resorption hormones, parathyroid hormone (PTH), suppressed the proliferation of cells and decreased the alkaline phosphatase activity at considerably low concentrations (1 X 10(-12)-1 X 10(-11) M). However, the hormone stimulated the incorporation of 45Ca2+ by these cells in a dose-dependent manner; the maximum stimulation on day 3 was observed at 1 X 10(-7) M and it was approximately 3 times the control value. The data suggest therefore, that the osteoblasts incorporated calcium ions and transported them while bone resorption was occurring. Thus the ROS 17/2.8 cell line appears to be an advantageous experimental system for the study of calcium metabolism of osteoblasts in vitro.

摘要

使用源自大鼠骨肉瘤的细胞系之一ROS 17/2.8培养细胞，研究了白细胞介素1、转化生长因子-β（偶联因子）、前列腺素E1和前列腺素E2对45Ca2+掺入及碱性磷酸酶活性的影响。我们发现所有这些因子均刺激这些细胞的45Ca2+掺入及碱性磷酸酶活性。另一方面，骨吸收激素之一甲状旁腺激素（PTH）在相当低的浓度（1×10−12 - 1×10−11 M）下抑制细胞增殖并降低碱性磷酸酶活性。然而，该激素以剂量依赖性方式刺激这些细胞对45Ca2+的掺入；在第3天，1×10−7 M时观察到最大刺激，约为对照值的3倍。因此，数据表明，在骨吸收发生时，成骨细胞摄取钙离子并进行转运。因此，ROS 17/2.8细胞系似乎是体外研究成骨细胞钙代谢的有利实验系统。