豚鼠皮肤中依赖于哈格曼因子的激肽生成系统：各成分的血管外定位，以及该系统抑制剂耗竭动物中延长的血管反应。

Hageman factor dependent kinin generation system in guinea pig skin: extravascular localization of the components, and prolonged vascular reaction in inhibitor-depleted animal of this system.

Yamamoto T, Ishimatsu T, Kambara T

Department of Allergy, Kumamoto University Medical School, Japan.

Adv Exp Med Biol. 1989;247A:447-52. doi: 10.1007/978-1-4615-9543-4_68.

Abstract

Monospecific antibodies against components of the guinea pig Hageman factor-dependent system, such as Hageman factor, prekallikrein and high-molecular-weight (HMW) kininogen were prepared. These antibodies demonstrated the presence of all of these components in the normal guinea pig skin extract with the properties identical to them in plasma on western immunoblotting analysis. 2. Guinea pig macroalbumin which is a major circulating inhibitor of activated Hageman factor was immunologically depleted from three animals. Duration of a vascular permeability reaction caused by an intradermal injection of activated Hageman factor was prolonged in all of these guinea pigs approximately twice as long as the control, indicating a presence of negative feedback regulation of the Hageman factor-dependent kinin generation cascade in dermal tissue via the circulating protease inhibitor.

摘要

制备了针对豚鼠接触因子依赖性系统成分的单特异性抗体，如接触因子、前激肽释放酶和高分子量（HMW）激肽原。这些抗体在western免疫印迹分析中证明，正常豚鼠皮肤提取物中存在所有这些成分，其性质与血浆中的相同。2. 从三只动物体内免疫清除了豚鼠巨白蛋白，它是活化接触因子的主要循环抑制剂。皮内注射活化接触因子引起的血管通透性反应持续时间在所有这些豚鼠中都延长了，大约是对照组的两倍，这表明通过循环蛋白酶抑制剂，在皮肤组织中存在接触因子依赖性激肽生成级联反应的负反馈调节。