Lara G H B, Takai S, Sasaki Y, Kakuda T, Listoni F J P, Risseti R M, de Morais A B C, Ribeiro M G
Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Universidade Estadual Paulista - UNESP, Botucatu, Brazil.
Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Japan.
Lett Appl Microbiol. 2015 Sep;61(3):306-10. doi: 10.1111/lam.12458. Epub 2015 Aug 2.
The virulence-plasmid profile of Rhodococcus equi strains isolated from Suidae and humans is similar. Recent evidence suggests that the consumption of pork products contaminated with faeces might be a potential source of R. equi infections in humans, mainly to patients with rhodococcosis without history of contact with pigs or pig farms. This study investigated the virulence-associated genes (vapA and vapB) and plasmid profiles of R. equi among the 150 samples of small intestinal content obtained from slaughtered pigs. In addition, all samples were subjected to microbiological culture in conventional sheep blood agar and CAZ-NB, TCP and TVP selective media. A total of 40 (26·7%) of the samples recovered R. equi, with two samples recovering isolates harbouring the VapB type 8 plasmid. Among the 150 pigs sampled herein, CAZ-NB was considered the best selective medium for the isolation of R. equi from faeces. Our results provide evidence that the contamination of slaughtered pig carcasses with pathogenic R. equi might occur through faeces, representing a public health concern. Furthermore, this study is the first description of R. equi strains carrying the VapB plasmid in the gut of pigs.
Intermediately virulent (VapB) is a common plasmid-type harboured by R. equi isolated from pigs and humans with AIDS. Curiously, humans with rhodococcosis usually have no history of contact with pigs or pig farms. Virulence-plasmid profile of 40 R. equi isolated among 150 small intestine content samples from pigs revelled two carrying isolates with the VapB type-8 plasmids. Moreover, comparison of three selective culture media shows that CAZ-NB was the best. Our results provide evidence that contamination of slaughtered pig carcasses with pathogenic R. equi might occur through faeces, representing a public health concern. Furthermore, R. equi carrying VapB type-8 plasmids types are described for the first time in the gut of the pig.
从猪科动物和人类中分离出的马红球菌菌株的毒力质粒谱相似。最近的证据表明,食用被粪便污染的猪肉产品可能是人类感染马红球菌的潜在来源,主要是针对没有接触过猪或猪场病史的红球菌病患者。本研究调查了从屠宰猪获得的150份小肠内容物样本中马红球菌的毒力相关基因(vapA和vapB)和质粒谱。此外,所有样本均在常规绵羊血琼脂以及CAZ-NB、TCP和TVP选择性培养基中进行微生物培养。总共40份(26.7%)样本中分离出了马红球菌,其中两份样本分离出了携带VapB 8型质粒的菌株。在本文采样的150头猪中,CAZ-NB被认为是从粪便中分离马红球菌的最佳选择性培养基。我们的结果表明,致病性马红球菌可能通过粪便污染屠宰猪的胴体,这是一个公共卫生问题。此外,本研究首次描述了猪肠道中携带VapB质粒的马红球菌菌株。
中等毒力(VapB)是从猪和患有艾滋病的人类中分离出的马红球菌常见的质粒类型。奇怪的是,患有红球菌病的人类通常没有接触过猪或猪场的病史。从150份猪小肠内容物样本中分离出的40株马红球菌的毒力质粒谱显示,有两株携带VapB 8型质粒。此外,对三种选择性培养基的比较表明,CAZ-NB是最好的。我们的结果表明,致病性马红球菌可能通过粪便污染屠宰猪的胴体,这是一个公共卫生问题。此外,首次在猪肠道中描述了携带VapB 8型质粒的马红球菌。