通过酶电泳数据的计算机辅助统计分析对细菌基因种进行鉴定

Characterization of bacterial genospecies by computer-assisted statistical analysis of enzyme electrophoretic data.

作者信息

Picard B, Goullet P, Bouvet P J, Decoux G, Denis J B

机构信息

Laboratoire de Microbiologie, Faculté de Medicine Xavier-Bichat, Université Paris, VII, France.

出版信息

Electrophoresis. 1989 Oct;10(10):680-5. doi: 10.1002/elps.1150101004.

DOI:10.1002/elps.1150101004

PMID:2612464

Abstract

A computer-assisted statistical treatment of the electrophoretic data obtained from the analysis of two dehydrogenases and 27 kinds of esterases produced by strains belonging to the taxonomically complex genus Acinetobacter is described. The 12 genospecies were clearly separated from each other by correspondence analysis. For each genospecies the distances of the strains from their barycenter were computed and typical isolates suitable for use as reference strains were determined. This approach is suitable for the systematic study of other procaryotic or eucaryotic organisms.

摘要

描述了一种对电泳数据进行计算机辅助统计处理的方法，这些电泳数据来自对不动杆菌属这一分类复杂的属中菌株产生的两种脱氢酶和27种酯酶的分析。通过对应分析，12个基因种被清晰地彼此区分开来。对于每个基因种，计算了菌株到其重心的距离，并确定了适合用作参考菌株的典型分离株。这种方法适用于对其他原核生物或真核生物进行系统研究。