受污水污染河口底栖蟹类携带产气荚膜梭菌的情况。
Carriage of Clostridium perfringens by benthic crabs in a sewage-polluted estuary.
作者信息
La Sala Luciano F, Redondo Leandro M, Díaz Carrasco Juan M, Pereyra Ana María, Farber Marisa, Jost Helen, Fernández-Miyakawa Mariano E
机构信息
GEKKO, Grupo de Estudios en Conservación y Manejo, Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, San Juan 670, 8000 Bahía Blanca, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Rivadavia 1917, 1033 Ciudad Autónoma de Buenos Aires, Argentina.
Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Calle Las Cabañas y Los Reseros s/n, Casilla de Correo 25, 1712 Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Rivadavia 1917, 1033 Ciudad Autónoma de Buenos Aires, Argentina.
出版信息
Mar Pollut Bull. 2015 Aug 15;97(1-2):365-372. doi: 10.1016/j.marpolbul.2015.05.066. Epub 2015 Jun 27.
The Estuary of Bahía Blanca (EBB), Argentina, is an important wetland under intense sewage pollution. We investigated the occurrence of Clostridium perfringens (CP) in populations of two benthic crabs (Neohelice granulata and Cyrtograpsus angulatus) and in sediment from the EBB. CP was found in 49.1% of the crabs and all of the isolates were identified as type A. The alpha (cpa) and enterotoxin (cpe) encoding genes were identified. Genetic analyses identified 13 novel sequence types, and found no clustering among isolates, suggesting that CP is not part of the crabs' commensal flora. CP carriage was 51 times more likely in crabs from the area nearest sewage outfalls compared with crabs from a reference site. Our in vitro experiments suggest that the carriage of CP in crabs is transient. The use of these benthic crabs as monitoring organisms of sewage pollution in coastal habitats is proposed.
阿根廷布兰卡湾河口（EBB）是一个受到严重污水污染的重要湿地。我们调查了两种底栖蟹（颗粒新海伦蟹和角突圆方蟹）种群以及EBB沉积物中产气荚膜梭菌（CP）的存在情况。在49.1%的蟹中发现了CP，所有分离株均被鉴定为A型。鉴定出了编码α毒素（cpa）和肠毒素（cpe）的基因。遗传分析确定了13种新的序列类型，且分离株之间未发现聚类现象，这表明CP不是蟹共生菌群的一部分。与来自参考地点的蟹相比，来自最靠近污水排放口区域的蟹携带CP的可能性高出51倍。我们的体外实验表明，蟹体内CP的携带是短暂的。建议将这些底栖蟹用作沿海栖息地污水污染的监测生物。
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